NDLI: Cleaved amplified polymorphic sequence markers in sugi, Cryptomeria japonica D. Don, and their locations on a linkage map

Content Provider	Springer Nature Link
Author	Iwata, H. Uji Ihara, T. Yoshimura, K. Nagasaka, K. Mukai, Y. Tsumura, Y.
Copyright Year	2001
Abstract	Sugi, Cryptomeria japonica D. Don, is one of the most important forestry species in Japan. We here report the development of 217 CAPS markers derived from sugi cDNA libraries. More than half of a set of STS markers produced could be converted into CAPS markers using restriction endonuclease analysis. Of the 217 markers, 71 showed different patterns of polymorphism when they were digested with a range of endonucleases and, in total, 347 polymorphisms were found in the various combinations of STSs and endonucleases. When the polymorphisms gave co-dominant patterns in a screening program, the polymorphic information content (PIC) used to evaluate the value of the polymorphisms was relatively high (0.33, on average) compared to the information yielded by commonly used markers, like isozymes. The results of a segregation analysis suggest that approximately 80% of the CAPS markers developed here will show co-dominant inheritance. From logistic regression analysis, the polymorphisms were found to be associated more strongly with intron than with exon regions. Sixty two markers were subsequently mapped on the previously reported linkage map, 15 of which showed abnormal segregation, presumably caused by linkage with lethal factors.
Starting Page	881
Ending Page	895
Page Count	15
File Format	PDF
ISSN	00405752
Journal	Theoretical and Applied Genetics
Volume Number	103
Issue Number	6
e-ISSN	14322242
Language	English
Publisher	Springer-Verlag
Publisher Date	2001-11-01
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Biotechnology Agronomy and Crop Science

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