NDLI: Leveraging the rice genome sequence for monocot comparative and translational genomics

Content Provider	Springer Nature Link
Author	Lohithaswa, H. C. Feltus, F. A. Singh, H. P. Bacon, C. D. Bailey, C. D. Paterson, A. H.
Copyright Year	2007
Abstract	Common genome anchor points across many taxa greatly facilitate translational and comparative genomics and will improve our understanding of the Tree of Life. To add to the repertoire of genomic tools applicable to the study of monocotyledonous plants in general, we aligned Allium and Musa ESTs to Oryza BAC sequences and identified candidate Allium-Oryza and Musa-Oryza conserved intron-scanning primers (CISPs). A random sampling of 96 CISP primer pairs, representing loci from 11 of the 12 chromosomes in rice, were tested on seven members of the order Poales and on representatives of the Arecales, Asparagales, and Zingiberales monocot orders. The single-copy amplification success rates of Allium (31.3%), Cynodon (31.4%), Hordeum (30.2%), Musa (37.5%), Oryza (61.5%), Pennisetum (33.3%), Sorghum (47.9%), Zea (33.3%), Triticum (30.2%), and representatives of the palm family (32.3%) suggest that subsets of these primers will provide DNA markers suitable for comparative and translational genomics in orphan crops, as well as for applications in conservation biology, ecology, invasion biology, population biology, systematic biology, and related fields.
Starting Page	237
Ending Page	243
Page Count	7
File Format	PDF
ISSN	00405752
Journal	Theoretical and Applied Genetics
Volume Number	115
Issue Number	2
e-ISSN	14322242
Language	English
Publisher	Springer-Verlag
Publisher Date	2007-05-24
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biotechnology Biochemistry Plant Biochemistry Plant Sciences Agriculture Plant Genetics & Genomics
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Biotechnology Agronomy and Crop Science

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