NDLI: Virus resistance in transgenic sweetpotato [Ipomoea batatas L. (Lam)] expressing the coat protein gene of sweet potato feathery mottle virus

Please wait, while we are loading the content...

Phylogenetic and genomic relationships in Setaria italica and its close relatives based on the molecular diversity and chromosomal organization of 5S and 18S-5.8S-25S rDNA genes

Relationships among Nicotiana species revealed by the 5S rDNA spacer sequence and fluorescence in situ hybridization

Establishment of a complete series of a monosomic tomato chromosome addition lines in the cultivated potato using RFLP and GISH analyses

Introgression of chromosomes of Festuca arundinacea var. glaucescens into Lolium multiflorum revealed by genomic in situ hybridisation (GISH)

Genetic mapping of QTLs conditioning soybean sprout yield and quality

’Forrest’ resistance to the soybean cyst nematode is bigenic: saturation mapping of the Rhg1and Rhg4 loci

The C1 locus in maize (Zea mays L.): effect on gene expression

Intra- and inter-specific variations in the mitochondrial gene orf138 of Ogura-type male-sterile cytoplasm from Raphanus sativus and Raphanus raphanistrum

Characterisation of two gene subunits on the 1R chromosome of rye as orthologs of each of the Glu-1 genes of hexaploid wheat

Virus resistance in transgenic sweetpotato [Ipomoea batatas L. (Lam)] expressing the coat protein gene of sweet potato feathery mottle virus

Identification of a mutant fatty acid elongase allele from zero-percent erucic acid Sinapis alba

Chromosomal location of a gene for resistance to septoria tritici blotch (Mycosphaerella graminicola)in the hexaploid wheat ’Synthetic 6x’

Genome organization in dicots. II. Arabidopsis as a ’bridging species’ to resolve genome evolution events among legumes

QTLs for agronomic traits from a Mediterranean barley progeny grown in several environments

Development of SCAR markers in olive (Olea europaea) by direct sequencing of RAPD products: applications in olive germplasm evaluation and mapping

Allozyme polymorphisms in tetraploid potato gene pools and the effect on human selection

Genetic mapping of gray leaf spot (GLS) resistance genes in maize

Power of the joint segregation analysis method for testing mixed major-gene and polygene inheritance models of quantitative traits

Theoretical and Applied Genetics : Volume 103, Issue 4, September 2001

Theoretical and Applied Genetics : Volume 103, Issue 2-3, August 2001

Theoretical and Applied Genetics : Volume 103, Issue 1, July 2001

Theoretical and Applied Genetics : Volume 102

Theoretical and Applied Genetics : Volume 101

Theoretical and Applied Genetics : Volume 100

Theoretical and Applied Genetics : Volume 99

Theoretical and Applied Genetics : Volume 98

Theoretical and Applied Genetics : Volume 97

Theoretical and Applied Genetics : Volume 96

Theoretical and Applied Genetics : Volume 95

Theoretical and Applied Genetics : Volume 94

Virus resistance in transgenic sweetpotato [Ipomoea batatas L. (Lam)] expressing the coat protein gene of sweet potato feathery mottle virus

Content Provider	Springer Nature Link
Author	Okada, Y. Saito, A. Nishiguchi, M. Kimura, T. Mori, M. Hanada, K. Sakai, J. Miyazaki, C. Matsuda, Y. Murata, T.
Copyright Year	2001
Abstract	One of the most-serious diseases of sweet potato [Ipomoea batatas (L.) Lam] is russet crack disease caused by sweet potato feathery mottle virus (SPFMV). We constructed an expression vector carrying the coat protein (CP) and hygromycin phosphotransferase (hpt) genes driven by cauliflower mosaic virus 35 S promoters. Accordingly, we introduced the expression vector into sweet potato variety Chikei 682-11 by the electroporation method. Among 449 calli obtained after antibiotic selection, 19 plants from seven independent calli grew to form adventitious shoots. Three transgenic lines were obtained from independent calli, based on analysis of the CP and hpt genes. The transcription and translation of the CP gene were shown in these transgenic lines by Northern- and Western-blot analyses. To assay the virus resistance of the transgenic lines, each line was vegetatively propagated and then grafted with morning glory (Ipomoea nil) that had been infected with SPFMV-S. A PAS-ELISA assay with polyclonal antiserum of the CP demonstrated that virus accumulation 3 months after grafting with the infected morning glory was suppressed in the transgenic lines as compared with non-transgenic ones. These transgenic lines were shown to be highly resistant not only to primary but also to secondary infection by SPFMV-S. Thus we concluded that the three transgenic lines with the CP gene of SPFMV-S can be used for coat protein-mediated resistance to the virus.
Starting Page	743
Ending Page	751
Page Count	9
File Format	PDF
ISSN	00405752
Journal	Theoretical and Applied Genetics
Volume Number	103
Issue Number	5
e-ISSN	14322242
Language	English
Publisher	Springer-Verlag
Publisher Date	2001-10-01
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Biotechnology Agronomy and Crop Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Analysis of transitive RNA silencing after grafting in transgenic plants with the coat protein gene of Sweet potato feathery mottle virus

Touch-induced gene (IbTCH1) from sweet potato [Ipomoea batatas (L.) Lam.]: molecular cloning and functional analysis

Homology-dependent virus resistance in transgenic plants with the coat protein gene of sweet potato feathery mottle potyvirus: target specificity and transgene methylation.

Cryopreservation of sweet potato (Ipomoea batatas [L.] Lam.) shoot tips by vitrification.

Comparisons among isolates of Sweet potato feathery mottle virus using complete genomic RNA sequences

A novel J8 domain gene,IbJ8, inIpomoea batatas (L). Lam.

Optimisation of somatic embryogenesis in fourteen cultivars of sweet potato [Ipomoea batatas (L.) Lam.]

Isozyme modifications and plant regeneration through somatic embryogenesis in sweet potato (Ipomoea batatas (L.) Lam.).

Comparisons of coat protein gene sequences show that East African isolates of Sweet potato feathery mottle virus form a genetically distinct group

Virus resistance in transgenic sweetpotato [Ipomoea batatas L. (Lam)] expressing the coat protein gene of sweet potato feathery mottle virus

Similar Documents

Analysis of transitive RNA silencing after grafting in transgenic plants with the coat protein gene of Sweet potato feathery mottle virus

Touch-induced gene (IbTCH1) from sweet potato [Ipomoea batatas (L.) Lam.]: molecular cloning and functional analysis

Homology-dependent virus resistance in transgenic plants with the coat protein gene of sweet potato feathery mottle potyvirus: target specificity and transgene methylation.

Cryopreservation of sweet potato (Ipomoea batatas [L.] Lam.) shoot tips by vitrification.

Comparisons among isolates of Sweet potato feathery mottle virus using complete genomic RNA sequences

A novel J8 domain gene,IbJ8, inIpomoea batatas (L). Lam.

Optimisation of somatic embryogenesis in fourteen cultivars of sweet potato [Ipomoea batatas (L.) Lam.]

Isozyme modifications and plant regeneration through somatic embryogenesis in sweet potato (Ipomoea batatas (L.) Lam.).

Comparisons of coat protein gene sequences show that East African isolates of Sweet potato feathery mottle virus form a genetically distinct group

Virus resistance in transgenic sweetpotato [Ipomoea batatas L. (Lam)] expressing the coat protein gene of sweet potato feathery mottle virus