NDLI: Development and genetic mapping of 127 new microsatellite markers in barley

Content Provider	Springer Nature Link
Author	Li, J. Z. Sjakste, T. G. Röder, M. S. Ganal, M. W.
Copyright Year	2003
Abstract	To enhance the marker density of existing genetic maps of barley (Hordeum vulgare L.), a new set of microsatellite markers containing dinucleotide motifs was developed from genomic clones. Out of 254 primer pairs tested, a total of 167 primer pairs were classifed as functional in a panel of six barley cultivars and three H. spontaneum accessions, and of those, 127 primer pairs resulting in 133 loci were either mapped or located onto chromosomes. The polymorphism information content (PIC) ranged from 0.05 to 0.94 with an average of 0.60. The number of alleles per locus varied from 1 to 9. On average, 3.9 alleles per primer pair were observed. The RFLP frameworks of two previously published linkage maps were used to locate a total of 115 new microsatellite loci on at least one mapping population. The chromosomal assignment of 48 mapped loci was corroborated on a set of wheat-barley chromosome addition lines; 18 additional loci which were not polymorphic in the mapping populations were assigned to chromosomes by this method. The microsatellites were located on all seven linkage groups with four significant clusters in the centromeric regions of 2H, 3H, 6H and 7H. These newly developed microsatellites improve the density of existing barley microsatellite maps and can be used in genetic studies and breeding research.
Starting Page	1021
Ending Page	1027
Page Count	7
File Format	PDF
ISSN	00405752
Journal	Theoretical and Applied Genetics
Volume Number	107
Issue Number	6
e-ISSN	14322242
Language	English
Publisher	Springer-Verlag
Publisher Date	2003-07-16
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Hordeum vulgare Linkage map SSRs Segregation distortion PIC
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Biotechnology Agronomy and Crop Science

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