NDLI: Identification and validation of molecular markers linked to the leaf rust resistance gene Lr19 in wheat

Content Provider	Springer Nature Link
Author	Gupta, Sudhir Kumar Charpe, Ashwini Prabhu, Kumble Vid Haque, Qazi Mohammad Rizwanul
Copyright Year	2006
Abstract	A leaf rust resistance gene Lr19 on the chromosome 7DL of wheat derived from Agropyron elongatum was tagged with random amplified polymorphic DNA (RAPD) and microsatellite markers. The F2 population of 340 plants derived from a cross between the leaf rust resistant near-isogenic line (NIL) of Thatcher (Tc + Lr19) and leaf rust susceptible line Agra Local that segregated for dominant monogenic leaf rust resistance was utilized for generating the mapping population. The molecular markers were mapped in the F2 derived F3 homozygous population of 140 seedlings. Sixteen RAPD markers were identified as linked to the alien gene Lr19 among which eight were in a coupling phase linkage. Twelve RAPD markers co-segregated with Lr19 locus. Nine microsatellite markers located on the long arm of chromosome 7D were also mapped as linked to the gene Lr19, including 7 markers which co-segregated with Lr19 locus, thus generating a saturated region carrying 25 molecular markers linked to the gene Lr19 within 10.2 ± 0.062 cM on either side of the locus. Two RAPD markers S265512 and S253737 which flanked the locus Lr19 were converted to sequence characterized amplified region markers SCS265512 and SCS253736, respectively. The marker SCS265512 was linked with Lr19 in a coupling phase and the marker SCS253736 was linked in a repulsion phase, which when used together mimicked one co-dominant marker capable of distinguishing the heterozygous resistant seedlings from the homozygous resistant. The molecular markers were validated on NILs mostly in Thatcher background isogenic for 44 different Lr genes belonging to both native and alien origin. The validation for polymorphism in common leaf rust susceptible cultivars also confirmed the utility of these tightly linked markers to the gene Lr19 in marker-assisted selection.
Starting Page	1027
Ending Page	1036
Page Count	10
File Format	PDF
ISSN	00405752
Journal	Theoretical and Applied Genetics
Volume Number	113
Issue Number	6
e-ISSN	14322242
Language	English
Publisher	Springer-Verlag
Publisher Date	2006-08-01
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biotechnology Biochemistry Plant Biochemistry Plant Sciences Agriculture Plant Genetics & Genomics
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Biotechnology Agronomy and Crop Science

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