NDLI: Development of PCR-based markers linked to dominant genes for male-fertility restoration in Pampa CMS of rye (Secale cereale L.)

Content Provider	Springer Nature Link
Author	Stracke, S. Schilling, A. G. Förster, J. Weiss, C. Glass, C. Miedaner, T. Geiger, H. H.
Copyright Year	2003
Abstract	Cytoplasmatic male sterility (CMS) is the basis for commercial hybrid seed production of rye. Nuclear restorer genes are indispensable for a complete restoration of fertility of the CMS lines. The drawbacks of current European restorer lines require the utilisation of new genetic resources that have been recently detected in an Iranian primitive rye population (IRAN IX) and an Argentinean landrace (Pico Gentario). The introgression of these effective restorer genes (Rfp1 and Rfp2, respectively) into breeding material can be facilitated by marker-assisted selection. Using two F2 populations based on crosses between the non-restorer inbred line Lo6 and the restorer IRAN IX, as well as Pico Gentario, RAPDs and AFLPs were screened and led to a closely linked marker set for each of these genes. The conversion of the closest markers into fragment-specific sequence-characterised amplified region (SCAR) markers resulted in flanking ranges of 2.9 cM (Rfp1) and 5.2 cM (Rfp2). The application of these markers in backcross programmes is discussed.
Starting Page	1184
Ending Page	1190
Page Count	7
File Format	PDF
ISSN	00405752
Journal	Theoretical and Applied Genetics
Volume Number	106
Issue Number	7
e-ISSN	14322242
Language	English
Publisher	Springer-Verlag
Publisher Date	2003-01-30
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Biotechnology Agronomy and Crop Science

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Mapping of genes for male-fertility restoration in ’Pampa’ CMS winter rye (Secale cereale L.)

Genetics and molecular mapping of a male fertility restoration locus (Rfg1) in rye (Secale cereale L.)

Molecular markers linked to the aluminium tolerance gene Alt1 in rye (Secale cereale L.)

AFLP markers tightly linked to the aluminum-tolerance gene Alt3 in rye (Secale cereale L.)

Mapping of three self-fertility mutations in rye (Secale cereale L.) using RFLP, isozyme and morphological markers

DArT markers tightly linked with the Rfc1 gene controlling restoration of male fertility in the CMS-C system in cultivated rye (Secale cereale L.)

Leaf-rust resistance in rye (Secale cereale L.). 1. Genetic analysis and mapping of resistance genes Pr1 and Pr2

Molecular linkage mapping in rye (Secale cereale L.)

Development of new PCR-based markers specific for chromosome arms of rye (Secale cereale L.).

Development of PCR-based markers linked to dominant genes for male-fertility restoration in Pampa CMS of rye (Secale cereale L.)

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Mapping of genes for male-fertility restoration in ’Pampa’ CMS winter rye (Secale cereale L.)

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