NDLI: Analysis of a dehydrin encoding gene and its phylogenetic utility in Helianthus

Content Provider	Springer Nature Link
Author	Giordani, T. Natali, L. Cavallini, A.
Copyright Year	2003
Abstract	Dehydrins are ubiquitous plant proteins, synthesized in late stages of plant embryo development and following any environmental stress involving dehydration. With the aim to study the evolution of such a stress-responsive gene within Helianthus and to test the possibility of using this gene for phylogenetic studies, fragments of the same dehydrin gene were isolated by PCR and sequenced in 16 wild Helianthus species or subspecies. All isolated sequences included the typical dehydrin domains (Y, S and K), a portion of 3′-UTR and an intron, inserted in the same position within the S domain-encoding region. The number of nucleotide substitutions (both synonymous and nonsynonymous) was calculated keeping separate the different gene regions, and differences occur even among coding domains, indicating that evolutionary constraints act differently on each region. The occurrence of indels and/or insertions was also observed. At the deduced protein level, the calculation of isoelectric point, molecular weight and the percentage of α-helix showed a diversification of biochemical properties of this protein between annual and perennial Helianthus species. Phylogenetic trees were built by the maximum-likelihood, maximum-parsimony, and neighbor-joining methods. In all cases the same topology was observed; perennial and annual species form a supported clade, and H. annuus was separated from the other annuals and from perennials. These data support the use of this stress-responsive gene to study the phylogeny of Helianthus.
Starting Page	316
Ending Page	325
Page Count	10
File Format	PDF
ISSN	00405752
Journal	Theoretical and Applied Genetics
Volume Number	107
Issue Number	2
e-ISSN	14322242
Language	English
Publisher	Springer-Verlag
Publisher Date	2003-04-23
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Dehydrin Helianthus Sunflower Indel Substitution Phylogenetic trees
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Biotechnology Agronomy and Crop Science

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