NDLI: A bacterial artificial chromosome library for sugarcane

Content Provider	Springer Nature Link
Author	Tomkins, J. P. Yu, Y. Miller Smith, H. Frisch, D. A. Woo, S. S. Wing, R. A.
Copyright Year	1999
Abstract	Modern cultivated sugarcane is a complex aneuploid polyploid with an estimated genome size of 3000 Mb. Although most traits in sugarcane show complex inheritance, a rust locus showing monogenic inheritance has been documented. In order to facilitate cloning of the rust locus, we have constructed a bacterial artificial chromosome (BAC) library for the cultivar R570. The library contains 103,296 clones providing 4.5 sugarcane genome equivalents. A random sampling of 240 clones indicated an average insert size of 130 kb allowing a 98% probability of recovering any specific sequence of interest. High-density filters were gridded robotically using a Genetix Q-BOT in a 4 × 4 double-spotted array on 22.5-cm2 filters. Each set of five filters provides a genome coverage of 4x with 18,432 clones represented per filter. Screening of the library with three different barley chloroplast gene probes indicated an exceptionally low chloroplast DNA content of less than 1%. To demonstrate the library’s potential for map-based cloning, single-copy RFLP sugarcane mapping probes anchored to nine different linkage groups and three different gene probes were used to screen the library. The number of positive hybridization signals resulting from each probe ranged from 8 to 60. After determining addresses of the signals, clones were evaluated for insert size and HindIII-fingerprinted. The fingerprints were then used to determine clone relationships and assemble contigs. For comparison with other monocot genomes, sugarcane RFLP probes were also used to screen a Sorghum bicolor BAC library and two rice BAC libraries. The rice and sorghum BAC clones were characterized for insert size and fingerprinted, and the results compared to sugarcane. The library was screened with a rust resistance RFLP marker and candidate BAC clones were subjected to RFLP fragment matching to identify those corresponding to the same genomic region as the rust gene.
Starting Page	419
Ending Page	424
Page Count	6
File Format	PDF
ISSN	00405752
Journal	Theoretical and Applied Genetics
Volume Number	99
Issue Number	3
e-ISSN	14322242
Language	English
Publisher	Springer-Verlag
Publisher Date	1999-08-24
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Plant Breeding/Biotechnology Plant Genetics & Genomics Agriculture Plant Biochemistry Biochemistry Biotechnology
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Biotechnology Agronomy and Crop Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

A ‘Chinese Spring’ wheat (Triticum aestivum L.) bacterial artificial chromosome library and its use in the isolation of SSR markers for targeted genome regions

Informative genomic microsatellite markers for efficient genotyping applications in sugarcane

Prospecting sugarcane resistance to Sugarcane yellow leaf virus by genome-wide association

Genetic control of yield related stalk traits in sugarcane

Bayesian estimation of marker dosage in sugarcane and other autopolyploids

Simple sequence repeat marker development from bacterial artificial chromosome end sequences and expressed sequence tags of flax (Linum usitatissimum L.)

Experimental assessment of the accuracy of genomic selection in sugarcane

Targeting xa13, a recessive gene for bacterial blight resistance in rice

A method for accelerated trait conversion in plant breeding

A bacterial artificial chromosome library for sugarcane

Similar Documents

A ‘Chinese Spring’ wheat (Triticum aestivum L.) bacterial artificial chromosome library and its use in the isolation of SSR markers for targeted genome regions

Informative genomic microsatellite markers for efficient genotyping applications in sugarcane

Prospecting sugarcane resistance to Sugarcane yellow leaf virus by genome-wide association

Genetic control of yield related stalk traits in sugarcane

Bayesian estimation of marker dosage in sugarcane and other autopolyploids

Simple sequence repeat marker development from bacterial artificial chromosome end sequences and expressed sequence tags of flax (Linum usitatissimum L.)

Experimental assessment of the accuracy of genomic selection in sugarcane

Targeting xa13, a recessive gene for bacterial blight resistance in rice

A method for accelerated trait conversion in plant breeding

A bacterial artificial chromosome library for sugarcane