NDLI: Molecular characterization of HMW glutenin subunit allele 1Bx14: further insights into the evolution of Glu-B1-1 alleles in wheat and related species

Content Provider	Springer Nature Link
Author	Li, W. Wan, Y. Liu, Z. Liu, K. Liu, X. Li, B. Li, Z. Zhang, X. Dong, Y. Wang, D.
Copyright Year	2004
Abstract	1Bx14 is a member of the high molecular weight (HMW) glutenin subunits specified by wheat Glu-B1-1 alleles. In this work, we found that the full-length amino acid sequence of 1Bx14 derived from cloned coding region was similar, but not identical, to that of 1Bx20. In the N-terminal domains of 1Bx14 and 1Bx20, the last two of the three cysteine residues, which are conserved in 1Bx7, 1Bx17 and homoeologous 1Ax and 1Dx subunits, were replaced by tyrosine residues. In the 5′ flanking regions (−900 to −1,200 bp relative to the start codon), a novel miniature inverted-repeat transposable element insertion was present in 1Bx14 and 1Bx20 but not 1Bx7 and 1Bx17. 1Bx14 and 1Bx20 like alleles were readily found in tetraploid wheat subspecies but not several S genome containing Aegilops species. Phylogenetic analysis showed that the four molecularly characterized Glu-B1-1 alleles (1Bx7, 1Bx14, 1Bx17, 1Bx20) could be divided into two allelic lineages. The lineage represented by 1Bx7 and 1Bx17 was more ancient than the one represented by 1Bx14 and 1Bx20. Combined, our data establish that 1Bx14 and 1Bx20 represent a novel subclass of Glu-B1-1 alleles. Based on current knowledge, potential mechanism involved in the differentiation of two Glu-B1-1 lineages is discussed.
Starting Page	1093
Ending Page	1104
Page Count	12
File Format	PDF
ISSN	00405752
Journal	Theoretical and Applied Genetics
Volume Number	109
Issue Number	5
e-ISSN	14322242
Language	English
Publisher	Springer-Verlag
Publisher Date	2004-07-29
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Biotechnology Agronomy and Crop Science

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