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Methylation-sensitive RFLPs: characterisation of two oil palm markers showing somaclonal variation-associated polymorphism

Content Provider	Springer Nature Link
Author	Jaligot, E. Beulé, T. Rival, A.
Copyright Year	2002
Abstract	The occurrence of "mantled" somaclonal variants (approx. 5%), which display alterations in floral organ structure, among populations of somatic embryo-derived oil palms (Elaeis guineensis Jacq.) currently hampers any scaling-up of clonal plant micropropagation. As a first step towards the identification of abnormality-discriminating markers, we have screened a set of 27 oil palm cDNA probes for methylation-sensitive restriction fragment length polymorphisms (RFLPs) using callus genomic DNA digested with the isoschizomeric enzymes MspI and HpaII. Only two probes (CPHO62 and -63) were found to differentiate reproducibly in two different genotypic backgrounds between nodular compact calli (NCC) and fast-growing calli (FGC), which generate 5% and 100% "mantled" plantlets, respectively. Comparative analyses were then conducted on DNA from inflorescences and leaves of normal and abnormal adult regenerants. With both probes, the observed methylation patterns were strongly clone-dependent and monomorphic with respect to the phenotype of the regenerants, except for the type-specific banding pattern obtained with the CPHO62 probe on material from the LMC3 clonal offspring. The results presented here mirror the higher difference in genomic DNA methylation observed between normal and abnormal embryogenic calli when compared to more differentiated plant material. Moreover, they reinforce the paramount interest of NCC and FGC callus lines as a material of choice in the search for early epigenetic markers of the "mantled" somaclonal variation. The potential use of methylation-sensitive RFLPs for the early detection of somaclonal variation at early stages of the micropropagation process is discussed.
Starting Page	1263
Ending Page	1269
Page Count	7
File Format	PDF
ISSN	00405752
Journal	Theoretical and Applied Genetics
Volume Number	104
Issue Number	8
e-ISSN	14322242
Language	English
Publisher	Springer-Verlag
Publisher Date	2002-04-26
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Biotechnology Agronomy and Crop Science

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Somaclonal variation in oil palm (Elaeis guineensis Jacq.): the DNA methylation hypothesis

Variation in oil palm (Elaeis guineensis Jacq.) tissue culture-derived regenerants revealed by AFLPs with methylation-sensitive enzymes

Retroelements, transposons and methylation status in the genome of oil palm (Elaeis guineensis) and the relationship to somaclonal variation

Variations in genomic DNA methylation during the long-term in vitro proliferation of oil palm embryogenic suspension cultures

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Morpho-histological characterization of truncated leaf syndrome seedlings: an oil palm (E. guineensis Jacq.) somaclonal variant

Application of RAPD markers in the characterisation of Chrysanthemum varieties and the assessment of somaclonal variation

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Effects of methylation-sensitive enzymes on the enrichment of genic SNPs and the degree of genome complexity reduction in a two-enzyme genotyping-by-sequencing (GBS) approach: a case study in oil palm (Elaeis guineensis)

Methylation-sensitive RFLPs: characterisation of two oil palm markers showing somaclonal variation-associated polymorphism

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Methylation-sensitive RFLPs: characterisation of two oil palm markers showing somaclonal variation-associated polymorphism