NDLI: Increase of fibronectin and osteopontin in porcine hearts following ischemia and reperfusion

Content Provider	Springer Nature Link
Author	Kossmehl, Peter Schönberger, Johann Shakibaei, Mehdi Faramarzi, Shideh Kurth, Ekkehard Habighorst, Britta Bauer, Rüdiger Wehland, Markus Kreutz, Reinhold Infanger, Manfred Schulze Tanzil, Gundula Paul, Martin Grimm, Daniela
Copyright Year	2005
Abstract	Following a severe ischemic injury or myocardial infarction, the extracellular matrix (ECM) of the heart is involved in pathophysiological conditions such as dilatation and cardiac dysfunction. Osteopontin (OPN) has been shown to interact with fibronectin suggesting its possible role in matrix organization, stability and wound healing. There is increased expression of OPN in several tissues in response to injury. Therefore, we tested the hypothesis that acute ischemia (2 h), followed by reperfusion (4 h) may induce early OPN and fibronectin in an isolated hemoperfused working porcine heart model. Twenty hearts were prepared and connected to a perfusion system. After 1 h of perfusion, these hearts were randomized to two groups: ten infarcted (MI, ramus circumflexus) and ten non-infarcted hearts (C). In addition, cardiac fibroblasts derived from infarcted, remote and control myocardium were investigated. In both groups, the heart rate, electrolytes, pH, blood gases, and lactate remained similar. The LVEDP and perfusion pressure of MI hearts increased significantly (P<0.05). The total fibronectin and OPN volume contents were clearly elevated in the infarct area. The matrix metalloproteinases (MMP-1 and MMP-8), fibronectin, OPN, TGF-β1 proteins and the mRNAs for fibronectin, TGF-β1, and OPN were significantly elevated in the infarct area as compared to the remote area and the non-infarcted hearts. Simultaneously, circulating carboxyterminal propeptide of type I procollagen (PICP) was released in the perfusion medium (threefold versus C). Fibroblast-like cells originating from the infarct area exhibited an enhanced OPN and fibronectin gene and protein expression compared to fibroblasts derived from control myocardium. Our data demonstrate the early appearance of the MMPs (increased collagen degrading enzymes) and PICP (a collagen synthesis marker) following ischemia and reperfusion. Moreover, OPN, fibronectin and TGF-β1 protein and gene expression are elevated after ischemia and reperfusion in the ex vivo working hemoperfused porcine heart model.
Starting Page	626
Ending Page	637
Page Count	12
File Format	PDF
ISSN	09462716
Journal	Journal of Molecular Medicine
Volume Number	83
Issue Number	8
e-ISSN	14321440
Language	English
Publisher	Springer-Verlag
Publisher Date	2005-03-16
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Ischemia Reperfusion Osteopontin Fibronectin Matrix metalloproteinases
Content Type	Text
Resource Type	Article
Subject	Drug Discovery Molecular Medicine Genetics (clinical)

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Structural studies of matrix metalloproteinases

The interaction between ischemia–reperfusion and immune responses in the kidney

Therapeutic approaches for ischemia/reperfusion injury in the liver

Matrix metalloproteinases and their regulators are cardiovascular therapeutic targets

A zinc transporter protects from ischemia–reperfusion injury

Zinc fingers protect the kidney from ischemia/reperfusion injury

NADPH oxidases as a source of oxidative stress and molecular target in ischemia/reperfusion injury

Elevated circulating levels of matrix metalloproteinases MMP-2 and MMP-9 in pseudoxanthoma elasticum patients

The laminated hearts

Increase of fibronectin and osteopontin in porcine hearts following ischemia and reperfusion

Similar Documents

Structural studies of matrix metalloproteinases

The interaction between ischemia–reperfusion and immune responses in the kidney

Therapeutic approaches for ischemia/reperfusion injury in the liver

Matrix metalloproteinases and their regulators are cardiovascular therapeutic targets

A zinc transporter protects from ischemia–reperfusion injury

Zinc fingers protect the kidney from ischemia/reperfusion injury

NADPH oxidases as a source of oxidative stress and molecular target in ischemia/reperfusion injury

Elevated circulating levels of matrix metalloproteinases MMP-2 and MMP-9 in pseudoxanthoma elasticum patients

The laminated hearts

Increase of fibronectin and osteopontin in porcine hearts following ischemia and reperfusion