NDLI: Genetic analysis of the murine μ opioid receptor: increased complexity of Oprm gene splicing

Please wait, while we are loading the content...

A commentary on Coronary artery disease. Genes, drugs and the agricultural connection by Ole Færgeman (ed) : Elsevier Science, Amsterdam, 2003, ISBN 444.51396-5

Human genome research in China

Manipulation of glycogen-synthase kinase-3 activity in KSHV-associated cancers

The Ro 60 kDa autoantigen: insights into cellular function and role in autoimmunity

Effective induction of immune tolerance by portal venous infusion with IL-10 gene-modified immature dendritic cells leading to prolongation of allograft survival

Genetic analysis of the murine μ opioid receptor: increased complexity of Oprm gene splicing

Day/night rhythms in gene expression of the normal murine heart

Functional characterization of the human atrial essential myosin light chain (hALC-1) in a transgenic rat model

Journal of Molecular Medicine : Volume 82, Issue 3, March 2004

Journal of Molecular Medicine : Volume 82, Issue 2, February 2004

Journal of Molecular Medicine : Volume 82, Issue 1, January 2004

Journal of Molecular Medicine : Volume 81

Journal of Molecular Medicine : Volume 80

Journal of Molecular Medicine : Volume 79

Journal of Molecular Medicine : Volume 78

Journal of Molecular Medicine : Volume 77

Journal of Molecular Medicine : Volume 76

Journal of Molecular Medicine : Volume 75

Genetic analysis of the murine μ opioid receptor: increased complexity of Oprm gene splicing

Content Provider	Springer Nature Link
Author	Kvam, Tor Morten Baar, Cecilie Rakvåg, Trude T. Kaasa, Stein Krokan, Hans E. Skorpen, Frank
Copyright Year	2004
Abstract	Evidence exists that μ analgesics such as morphine, methadone and fentanyl may act through distinct μ opioid receptor mechanisms. It has been proposed that the functional diversity of μ opioid receptors may be related to alternative splicing of the Oprm gene. Although a number of μ opioid receptor mRNA splice variants have been reported, their biological relevance has been controversial, due in part to their very low abundance and a general lack of validation from independent laboratories. We have identified 11 of 17 proposed exons as well as the majority of exon combinations used to make 21 differentially spliced Oprm mRNAs from mouse whole brain cDNA, using polymerase chain reaction (PCR) conditions different from those used by the single other group that has reported multiple splice forms. Alternative splicing was shown to occur at both the 5′ and 3′ termini. Moreover, verification of a short variant, containing exons 1 and 4 only, suggests that splicing also occurs directly between 5′ and 3′ exons. Notably, a novel splice variant, MOR-1T, demonstrates for the first time that exon 4 can be used in combination with further downstream exons to make the 3′-end of MOR-1 splice variants. The putative protein encoded by MOR-1T is predicted to be identical to that of MOR-1, implying that the MOR-1 protein can be generated from at least five differentially spliced mRNAs. Our results support the view that the Oprm gene undergoes extensive alternative splicing, as a likely major contributor to the diversity of μ opioid receptors.
Starting Page	250
Ending Page	255
Page Count	6
File Format	PDF
ISSN	09462716
Journal	Journal of Molecular Medicine
Volume Number	82
Issue Number	4
e-ISSN	14321440
Language	English
Publisher	Springer-Verlag
Publisher Date	2004-01-09
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Mu opioid receptor Alternative splicing Splice variants
Content Type	Text
Resource Type	Article
Subject	Drug Discovery Molecular Medicine Genetics (clinical)

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Generation of the mu opioid receptor (MOR-1) protein by three new splice variants of the Oprm gene.

Generation of the mu opioid receptor (MOR-1) protein by three new splice variants of the Oprm gene

Exploring Pharmacological Functions of Alternatively Spliced Variants of the Mu Opioid Receptor Gene, Oprm1, via Gene-Targeted Animal Models

Alternative Pre-mRNA Splicing of the Mu Opioid Receptor Gene, OPRM1: Insight into Complex Mu Opioid Actions

Intragenic motifs regulate the transcriptional complexity of Pkhd1/PKHD1

Synthesis and evaluation of aryl-naloxamide opiate analgesics targeting truncated exon 11-associated mu opioid receptor (MOR-1) splice variants

Stabilization of the μ-Opioid Receptor by Truncated Single Transmembrane Splice Variants through a Chaperone-like Action

Heterogeneity of mu-opioid receptors as one of the factors sensitive to narcotic analgesics

Increased transcript diversity: novel splicing variants of Machado–Joseph Disease gene (ATXN3)

Genetic analysis of the murine μ opioid receptor: increased complexity of Oprm gene splicing

Similar Documents

Generation of the mu opioid receptor (MOR-1) protein by three new splice variants of the Oprm gene.

Generation of the mu opioid receptor (MOR-1) protein by three new splice variants of the Oprm gene

Exploring Pharmacological Functions of Alternatively Spliced Variants of the Mu Opioid Receptor Gene, Oprm1, via Gene-Targeted Animal Models

Alternative Pre-mRNA Splicing of the Mu Opioid Receptor Gene, OPRM1: Insight into Complex Mu Opioid Actions

Intragenic motifs regulate the transcriptional complexity of Pkhd1/PKHD1

Synthesis and evaluation of aryl-naloxamide opiate analgesics targeting truncated exon 11-associated mu opioid receptor (MOR-1) splice variants

Stabilization of the μ-Opioid Receptor by Truncated Single Transmembrane Splice Variants through a Chaperone-like Action

Heterogeneity of mu-opioid receptors as one of the factors sensitive to narcotic analgesics

Increased transcript diversity: novel splicing variants of Machado–Joseph Disease gene (ATXN3)

Genetic analysis of the murine μ opioid receptor: increased complexity of Oprm gene splicing