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Adaptation of a four-arm olfactometer for behavioural bioassays of large beetles

Content Provider	Springer Nature Link
Author	Saïd, Imen Torre, Rosa Aldana de la Morin, Jean Paul Rochat, Didier
Copyright Year	2005
Abstract	We adapted a four-arm olfactometer (55 × 55 × 5.5 cm) for bioassays of large insects and its usefulness was evaluated by testing the responses of three beetles, the palm weevil Rhynchophorus palmarum (L.) (Coleoptera: Curculionidea) and two Dynast scarabs Strategus aloeus (L.) and Oryctes rhinoceros (L.) (Coleoptera: Scarabaeidae) to plant odours and aggregation pheromones. This olfactometer was coupled to a volatile delivery system dispensing highly volatile semiochemicals at constant concentrations. We materialized airflows in the olfactometer by discolouring sensitive papers with acid vapours under various conditions (air straighten by stainless steel grids in inputs and output, 500 ml/min per arm) to visualize air turbulence and to test air-tightness. The volatile delivery system can be used to mix and dispense a broad range of concentrations of semiochemicals in air from diluted aqueous solutions. It was calibrated by measuring the release of the pheromone using solid phase-microextraction (SPME). Dose-response curves to synthetic pheromone were obtained for O. rhinoceros of both sexes. Coupling the volatile delivery system to the fourarm olfactometer provided a system with which the responses of R. palmarum and S. aloeus to their synthetic aggregation pheromones and to natural plant volatiles could be readily observed and studied.
Starting Page	9
Ending Page	16
Page Count	8
File Format	PDF
ISSN	09377409
Journal	CHEMOECOLOGY
Volume Number	16
Issue Number	1
e-ISSN	14230445
Language	English
Publisher	Birkhäuser-Verlag
Publisher Date	2005-10-25
Publisher Place	Basel
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Four-arm olfactometer volatile delivery system aggregation pheromone palm beetles Coleoptera Curculionidae Scarabaeidae Rhynchophorus palmarum Oryctes rhinoceros Strategus aloeus Nature Conservation Ecology
Content Type	Text
Resource Type	Article
Subject	Biochemistry Ecology, Evolution, Behavior and Systematics

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Adaptation of a four-arm olfactometer for behavioural bioassays of large beetles