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| Content Provider | Springer Nature Link |
|---|---|
| Author | Adlakha, Yogita K. Saini, Neeru |
| Copyright Year | 2010 |
| Abstract | MicroRNAs (miRNAs) are short ~21-nt non-coding RNA molecules that have been shown to regulate a number of biological processes. Previous reports have shown that overexpression of miR-128 in glioma cells inhibited cell proliferation. Literature also suggests that miR-128 negatively regulates prostate cancer cell invasion. Here, we show that overexpression of hsa-miR-128, a brain-enriched microRNA, induces apoptosis in HEK293T cells as elucidated by apoptosis assay, cell cycle changes, loss of mitochondrial membrane potential and multicaspase assay. By in silico analysis, we identified a putative target site within the 3′ untranslated region (UTR) of Bax, a proapoptotic member of the apoptosis pathway. We found that ectopic expression of hsa-miR-128 suppressed a luciferase reporter containing the Bax-3′ UTR and reduced the levels of Bax in HEK293T cells. Taken together, our study demonstrates that overexpression of hsa-miR-128 not only induces apoptosis in HEK293T cells but also is an endogenous regulator of Bax protein. |
| Starting Page | 1415 |
| Ending Page | 1428 |
| Page Count | 14 |
| File Format | |
| ISSN | 1420682X |
| Journal | Cellular and Molecular Life Sciences |
| Volume Number | 68 |
| Issue Number | 8 |
| e-ISSN | 14209071 |
| Language | English |
| Publisher | SP Birkhäuser Verlag Basel |
| Publisher Date | 2010-09-19 |
| Publisher Place | Basel |
| Access Restriction | Subscribed |
| Subject Keyword | miRNA Apoptosis Bax Reactive oxygen species Mitochondrial membrane potential Cell Biology Biochemistry Life Sciences Biomedicine general |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Molecular Biology Molecular Medicine Pharmacology Cellular and Molecular Neuroscience |
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