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| Content Provider | Springer Nature Link |
|---|---|
| Author | Torossi, T. Roth, J. Ziak, M. |
| Copyright Year | 2007 |
| Abstract | Golgi-endomannosidase provides an alternate glucosidase-independent pathway of glucose trimming. Activity for endomannosidase is detectable in various tissues and cell lines but not in CHO cells. Cloning of CHO cell endomannosidase revealed that the highly conserved Trp188 and Arg177 of vertebrate endomannosidase were both substituted by Cys. The Trp188Cys substitution was functionally important since it alone resulted in endoplasmic reticulum (ER) mislocalization of endomannosidase and caused the greatly reduced in vivo activity. These effects could be reversed in cells with a back-engineered Cys188Trp CHO cell endomannosidase, in particular N-glycans of α1-antitrypsin became fully processed. The intramolecular disulfide bridge of CHO cell endomannosidase formed with the additional Cys188 was not solely responsible for the reduced enzyme activity since endomannosidase with engineered Cys188Ala or Ser substitutions did not restore enzyme activity and was ER mislocalized. Thus, the conserved Trp188 residue in endomannosidases is of critical importance for correct subcellular localization and in vivo activity of the enzyme. |
| Starting Page | 1881 |
| Ending Page | 1889 |
| Page Count | 9 |
| File Format | |
| ISSN | 1420682X |
| Journal | Cellular and Molecular Life Sciences |
| Volume Number | 64 |
| Issue Number | 14 |
| e-ISSN | 14209071 |
| Language | English |
| Publisher | Birkhäuser-Verlag |
| Publisher Date | 2007-06-26 |
| Publisher Place | Basel |
| Access Restriction | Subscribed |
| Subject Keyword | Endomannosidase Golgi apparatus endoplasmic reticulum N-glycosylation α1-antitrypsin CHO Lec 23 cells Cell Biology Biomedicine general Life Sciences Biochemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Molecular Biology Molecular Medicine Pharmacology Cellular and Molecular Neuroscience |
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