NDLI: Lysosomal chymotrypsin B potentiates apoptosis via cleavage of Bid

Content Provider	Springer Nature Link
Author	Zhao, Kai Zhao, Xingyu Tu, Yaping Miao, Qi Cao, Dongxu Duan, Wenjuan Sun, Yang Wang, Jincheng Wei, Taotao Yang, Fuyu
Copyright Year	2010
Abstract	We have reported that chymotrypsin B (CtrB) is not just a digestive enzyme but is also stored in lysosomes. Herein, we demonstrated a broad distribution of CtrB and explored the involvement of CtrB in apoptosis. Exposure of RH-35 cells to H2O2 or palmitate induced the redistribution of lysosomal CtrB into the cytoplasm as a result of lysosomal membrane permeabilization (LMP). Suppression of CtrB significantly blocked apoptosis, while overexpression of CtrB sensitized apoptosis markedly. CtrB could cleave Bid under neutral conditions. In RH-35 cells with Bid silenced, apoptosis induced by CtrB protein was attenuated, suggesting that CtrB mediates apoptosis of RH-35 cells mainly through processing Bid. Our data also suggest that LMP occurs earlier than mitochondrial outer membrane permeabilization; Bid activation initiated by caspase-8 might be reinforced by CtrB in consequence of LMP, which causes a positive feedback loop leading to the accumulation of tBid, and results in lysosome- and mitochondrion-dependent apoptosis.
Starting Page	2665
Ending Page	2678
Page Count	14
File Format	PDF
ISSN	1420682X
Journal	Cellular and Molecular Life Sciences
Volume Number	67
Issue Number	15
e-ISSN	14209071
Language	English
Publisher	SP Birkhäuser Verlag Basel
Publisher Date	2010-04-02
Publisher Place	Basel
Access Restriction	Subscribed
Subject Keyword	Chymotrypsin B Lysosome Mitochondrion Apoptosis Bid Biochemistry Life Sciences Biomedicine general Cell Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Molecular Biology Molecular Medicine Pharmacology Cellular and Molecular Neuroscience

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