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Content Provider | Springer Nature Link |
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Author | Oddi, S. Bari, M. Battista, N. Barsacchi, D. Cozzani, I. Maccarrone, M. |
Copyright Year | 2005 |
Abstract | The signaling activity of anandamide (AEA) is terminated by its uptake across the cellular membrane and subsequent intracellular hydrolysis by the fatty acid amide hydrolase (FAAH). To date, the existence of an AEA membrane transporter (AMT) independent of FAAH activity remains questionable, although it has been recently corroborated by pharmacological and genetic data. We performed confocal microscopy and biochemical analysis in human HaCaT keratinocytes, in order to study the cellular distribution of AMT and FAAH. We found that FAAH is intracellularly localized as a punctate staining partially overlapping with the endoplasmic reticulum. Consistently, subcellular fractionation and reconstitution of vesicles from membranes of different compartments demonstrated that FAAH activity was localized mainly in microsomal fractions, whereas AMT activity was almost exclusively in plasma membranes. These results provide the first morphological and biochemical evidence to support the view that transport and hydrolysis are two spatially and functionally distinct processes in AEA degradation. |
Starting Page | 386 |
Ending Page | 395 |
Page Count | 10 |
File Format | |
ISSN | 1420682X |
Journal | Cellular and Molecular Life Sciences |
Volume Number | 62 |
Issue Number | 3 |
e-ISSN | 14209071 |
Language | English |
Publisher | Birkhäuser-Verlag |
Publisher Date | 2005-01-01 |
Publisher Place | Basel |
Access Restriction | Subscribed |
Subject Keyword | Differentiation endocannabinoid fatty acid amide hydrolase HaCaT cell lipid raft membrane transport Biomedicine general Life Sciences Biochemistry Cell Biology |
Content Type | Text |
Resource Type | Article |
Subject | Cell Biology Molecular Biology Molecular Medicine Pharmacology Cellular and Molecular Neuroscience |
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