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| Content Provider | Springer Nature Link |
|---|---|
| Author | Boudra, Mohammed Tayyib Bolin, Celeste Chiker, Sara Fouquin, Alexis Zaremba, Tomasz Vaslin, Laurence Biard, Denis Cordelières, Fabrice P. Mégnin Chanet, Frédérique Favaudon, Vincent Fernet, Marie Pennaneach, Vincent Hall, Janet |
| Copyright Year | 2014 |
| Abstract | Poly(ADP-ribose) polymerase-2 (PARP-2) activity contributes to a cells’ poly(ADP-ribosyl)ating potential and like PARP-1, has been implicated in several DNA repair pathways including base excision repair and DNA single strand break repair. Here the consequences of its stable depletion in HeLa, U20S, and AS3WT2 cells were examined. All three PARP-2 depleted models showed increased sensitivity to the cell killing effects on ionizing radiation as reported in PARP-2 depleted mouse embryonic fibroblasts providing further evidence for a role in DNA strand break repair. The PARP-2 depleted HeLa cells also showed both higher constitutive and DNA damage-induced levels of polymers of ADP-ribose (PAR) associated with unchanged PARP-1 protein levels, but higher PARP activity and a concomitant lower PARG protein levels and activity. These changes were accompanied by a reduced maximal recruitment of PARP-1, XRCC1, PCNA, and PARG to DNA damage sites. This PAR-associated phenotype could be reversed in HeLa cells on re-expression of PARP-2 and was not seen in U20S and AS3WT2 cells. These results highlight the complexity of the relationship between different members of the PARP family on PAR metabolism and suggest that cell model dependent phenotypes associated with the absence of PARP-2 exist within a common background of radiation sensitivity. |
| Starting Page | 1585 |
| Ending Page | 1597 |
| Page Count | 13 |
| File Format | |
| ISSN | 1420682X |
| Journal | Cellular and Molecular Life Sciences |
| Volume Number | 72 |
| Issue Number | 8 |
| e-ISSN | 14209071 |
| Language | English |
| Publisher | Springer Basel |
| Publisher Date | 2014-10-22 |
| Publisher Place | Basel |
| Access Restriction | Subscribed |
| Subject Keyword | Base excision repair Double strand break repair Single strand break repair Poly(ADP-ribose) polymerases PARG Ionizing Radiation Neocarzinostatin Cell Biology Biomedicine general Life Sciences Biochemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Molecular Biology Molecular Medicine Pharmacology Cellular and Molecular Neuroscience |
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