NDLI: Blockade of proteinase-activated receptor 4 inhibits neutrophil recruitment in experimental inflammation in mice

Content Provider	Springer Nature Link
Author	Gomides, Lindisley F. Lima, Onésia C. O. Matos, Natália A. Freitas, Kátia M. Francischi, Janetti gueira Tavares, Juliana Carvalho Klein, André
Copyright Year	2014
Abstract	The activation of proteinase-activated receptors (PARs) has been implicated in the development of important hallmarks of inflammation, including in vivo leukocyte recruitment; however, its role in the regulation of leukocyte migration in response to inflammatory stimuli has not been elucidated until now. Here, we examined the effects of the PAR4 antagonist YPGKF-NH 2 (tcY-NH2) on neutrophil recruitment in experimentally induced inflammation.BALB/c mice were intrapleurally injected with tcY-NH2 (40 ng/kg) prior to intrapleural injection of carrageenan (Cg) or neutrophil chemoattractant CXCL8; the number of infiltrating neutrophils was evaluated after 4 h, and KC production was assessed at different times after Cg injection. Neutrophil adhesion and rolling cells were studied using a brain circulation preparation 4 h after the Cg or CXCL8 challenge in tcY-NH2-treated mice.PAR4 blockade inhibited CXCL8- and Cg-induced neutrophil migration into the pleural cavity of BALB/c mice and reduced neutrophil rolling and adherence. Surprisingly, PAR4 blockade increased the level of KC in response to carrageenan.These results demonstrated that PAR4 blockade impairs neutrophil migration in vivo, suggesting that PAR4 plays an important role in the regulation of inflammation, at least in part because of its ability to inhibit the actions of the neutrophil chemoattractant CXCL8.
Starting Page	935
Ending Page	941
Page Count	7
File Format	PDF
ISSN	10233830
Journal	Inflammation Research
Volume Number	63
Issue Number	11
e-ISSN	1420908X
Language	English
Publisher	Springer Basel
Publisher Date	2014-08-14
Publisher Place	Basel
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Proteinase-activated receptor 4 Neutrophil migration CXCL8 Pleurisy Immunology Pharmacology/Toxicology Rheumatology Allergology Dermatology Neurology
Content Type	Text
Resource Type	Article
Subject	Pharmacology Immunology

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