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| Content Provider | Springer Nature Link |
|---|---|
| Author | Fang, Xiaoyun Hu, Hua Xie, Jianhui Zhu, Haiyan Zhang, Dongmei Mo, Wei Zhang, Ruxin Yu, Min |
| Copyright Year | 2012 |
| Abstract | To determine whether the neurokinin-1 receptor (NK1R) plays a role in the activation of RBL-2H3 mast cells after FcεRΙ aggregation.NK1R expression in RBL-2H3 cells was inhibited by small hairpin RNA (shRNA) against NK1R, and determined by western blotting. For activation, both NK1R knockdown and control RBL-2H3 cells were sensitized by dinitrophenol (DNP)-specific IgE and stimulated with the antigen DNP-bovine serum albumin (BSA). Following the activation of RBL-2H3 cells, monocyte chemoattractant protein (MCP-1) production and intracellular calcium flux were monitored by ELISA and confocal microscopy assay, respectively. For investigation of the signaling mechanism, phosphorylation of mitogen-activated protein kinases (MAPKs) after RBL-2H3 cell activation was assessed by western blotting.shRNA-NK1R mediated an effective inhibition of NK1R expression in RBL-2H3 cells. Protein production of MCP-1 was reduced by more than 55 % in NK1R knockdown RBL-2H3 cells compared with control RBL-2H3 cells. In addition, both calcium mobilization and phosphorylation levels of MAPKs (Erk1/2, JNK, and p38) after DNP-BSA stimulation (via FcεRΙ) were decreased due to the inhibition of NK1R expression.NK1R is required for the activation of RBL-2H3 cells following FcεRΙ engagement and involved in the regulation of MAPK signaling pathways. |
| Starting Page | 1257 |
| Ending Page | 1263 |
| Page Count | 7 |
| File Format | |
| ISSN | 10233830 |
| Journal | Inflammation Research |
| Volume Number | 61 |
| Issue Number | 11 |
| e-ISSN | 1420908X |
| Language | English |
| Publisher | SP Birkhäuser Verlag Basel |
| Publisher Date | 2012-07-21 |
| Publisher Place | Basel |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Neurokinin-1 receptor Mast cell FcεRΙ Signaling pathway Neurology Dermatology Immunology Allergology Pharmacology/Toxicology Rheumatology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Pharmacology Immunology |
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