NDLI: Superoxide production and NADPH oxidase expression in human rheumatoid synovial cells: regulation by interleukin-1β and tumour necrosis factor-α

Content Provider	Springer Nature Link
Author	Chenevier Gobeaux, C. Lemarechal, H. Bonnefont Rousselot, D. Poiraudeau, S. Ekindjian, O. G. Borderie, D.
Copyright Year	2006
Abstract	to evaluate the rheumatoid synovial cell capacity to produce superoxide anion in response to interleukin-1β (IL-1β) and tumour necrosis factor-α (TNF-α), and to study the NADPH oxidase involvement in this production.Synovial cells obtained from 7 rheumatoid arthritis (RA), 5 osteoarthritic (OA) patients, and dermal fibroblasts, were stimulated (i) with IL-1β and TNF-α, or (ii) with specific oxidase activators and inhibitors, before studying superoxide production; we also studied NADPH oxidase mRNAs and protein expression, and p47-phox phosphorylation.Constitutive superoxide production by RA cells was increased in comparison to OA cells and dermal fibroblasts, and was stimulated by PMA and ionomycin. This production was increased after cytokine treatment of RA synovial cells. Cytokine-induced superoxide production by RA cells was inhibited by iodonium diphenyl or apocynin, suggesting the involvement of NADPH oxidase. RT-PCR and western blot analysis revealed the presence of p47-phox, gp91-phox and Nox4 in RA and OA cells, and in dermal fibroblasts. P47-phox phosphorylation was enhanced after cytokine-treatment in RA and OA cells, suggesting a PKC-mediated up-regulation of NADPH oxidase.NADPH oxidase is involved in the superoxide release by RA synovial cells, constitutively and after cytokine up-regulation. These cells express two different homologues (gp91-phox and Nox4).
Starting Page	483
Ending Page	490
Page Count	8
File Format	PDF
ISSN	10233830
Journal	Inflammation Research
Volume Number	55
Issue Number	11
e-ISSN	1420908X
Language	English
Publisher	Birkhäuser-Verlag
Publisher Date	2006-01-01
Publisher Place	Basel
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	NADPH oxidase Superoxide anion Rheumatoid arthritis Synovial cells Immunology Pharmacology/Toxicology Allergology Dermatology Neurology Rheumatology
Content Type	Text
Resource Type	Article
Subject	Pharmacology Immunology

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