NDLI: MiRNA-140 is a negative feedback regulator of MMP-13 in IL-1β-stimulated human articular chondrocyte C28/I2 cells

Content Provider	Springer Nature Link
Author	Liang, Zu jian Zhuang, Hong Wang, Guo xia Li, Zhao Zhang, Huan tian Yu, Tian qi Zhang, Bai dang
Copyright Year	2012
Abstract	Osteoarthritis is a degenerative joint disease, in which matrix metalloproteinase (MMP)-13 plays an important role. This study aimed to investigate miRNA-140-mediated negative regulation of MMP-13 expression in interleukin-1β (IL-1β)-stimulated human cartilage cells.The human cartilage cell line C28/I2 was cultured in the presence of IL-1β to mimic an osteoarthritic environment. Expression of miRNA-140 and MMP-13 was analyzed after 48 h by real-time RT-PCR and western blot analyses. MiRNA-140 mediated regulation of MMP-13 expression was analyzed by luciferase reporter assays and anti-miRNA-140 oligonucleotide transfection. Furthermore, miRNA-140 and MMP-13 expression was analyzed following DHMEQ treatment.Expression of miRNA-140 and MMP-13 was elevated in IL-1β-stimulated C28/I2 cells. Bioinformatic prediction showed that the 3′-UTR of MMP-13 mRNA contained a potential binding miRNA-140 site and luciferase mRNA fused with 3′-UTR of MMP-13 mRNA was shown to be repressed by miRNA-140 in reporter assays. Expression of MMP-13 was elevated in IL-1β-stimulated C28/I2 cells following anti-miRNA-140 oligonucleotide transfection. NF-κB activity was inhibited in DHMEQ treated IL-1β-stimulated C28/I2 cells and was associated with decreased miRNA-140 and MMP-13 expression.Expression of miRNA-140 and MMP-13 was induced by IL-1β. Expression of miRNA-140 inhibited MMP-13 in C28/I2 cells. Expression of miRNA-140 and MMP-13 was shown to be NF-κB-dependent.
Starting Page	503
Ending Page	509
Page Count	7
File Format	PDF
ISSN	10233830
Journal	Inflammation Research
Volume Number	61
Issue Number	5
e-ISSN	1420908X
Language	English
Publisher	SP Birkhäuser Verlag Basel
Publisher Date	2012-01-25
Publisher Place	Basel
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	MiRNA-140 MMP-13 Osteoarthritis NF-κB Immunology Allergology Neurology Pharmacology/Toxicology Dermatology Rheumatology
Content Type	Text
Resource Type	Article
Subject	Pharmacology Immunology

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