NDLI: Adenosine effectively restores endotoxin-induced inhibition of human neutrophil chemotaxis via A1 receptor-p38 pathway

Content Provider	Springer Nature Link
Author	Xu, Xiaohan Zheng, Shuyun Xiong, Yuyun Wang, Xu Qin, Weiting Zhang, Huafeng Sun, Bingwei
Copyright Year	2017
Abstract	Neutrophil chemotaxis plays an essential role in recruiting neutrophils to sites of inflammation. Neutrophil chemotaxis is suppressed both after exposure to lipopolysaccharide (LPS) in vitro and during clinical and experimental endotoxemia, leading to serious consequences. Adenosine (ADO) is a potent anti-inflammatory agent that acts on a variety of neutrophil functions. However, its effects on human neutrophil chemotaxis during infection have been less well characterized. In the present study, we investigated the effect of ADO and its receptor-specific antagonist and agonist on neutrophil chemotaxis in an in vitro LPS-stimulated model. The results showed that increasing the concentration of ADO effectively restored the LPS-inhibited neutrophil chemotaxis to IL-8. A similar phenomenon occurred after intervention with a selective A1 receptor agonist but not with a selective antagonist. Pre-treatment with cAMP antagonist failed to restore LPS-inhibited chemotaxis. Furthermore, protein array and western blot analysis showed that the activation of A1 receptor significantly decreased LPS-induced p38 MAPK phosphorylation. However, the surface expression of the A1 receptor in LPS-stimulated neutrophils was not significantly changed. Taken together, these data indicated that ADO restored the LPS-inhibited chemotaxis via the A1 receptor, which downregulated the phosphorylation level of p38 MAPK, making this a promising new therapeutic strategy for infectious diseases.
Starting Page	353
Ending Page	364
Page Count	12
File Format	PDF
ISSN	10233830
Journal	Inflammation Research
Volume Number	66
Issue Number	4
e-ISSN	1420908X
Language	English
Publisher	Springer International Publishing
Publisher Date	2017-01-10
Publisher Place	Cham
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Neutrophil Adenosine Chemotaxis Lipopolysaccharide P38 Immunology Pharmacology/Toxicology Rheumatology Allergology Dermatology Neurology
Content Type	Text
Resource Type	Article
Subject	Pharmacology Immunology

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