NDLI: Chlamydia trachomatis heat shock proteins 60 and 10 induce apoptosis in endocervical epithelial cells

Content Provider	Springer Nature Link
Author	Jha, Rajneesh Vardhan, Harsh Bas, Sylvette Salhan, Sudha Mittal, Aruna
Copyright Year	2010
Abstract	The potential role of chlamydial heat shock proteins (cHSP) 60 and cHSP10 in apoptosis of primary cervical epithelial cells was investigated.Primary cervical epithelial cells were stimulated with cHSP60 and cHSP10 for 4 h. Quantitative measurements of apoptosis were made using cytofluorometry, and apoptosis-related genes were analyzed by microarray, real-time PCR and western blotting. Further, levels of proinflammatory cytokines (IL-18 and IL-1β) were determined by semi-quantitative RT-PCR.After a 4-h incubation in the presence of recombinant cHSP60 or cHSP10, the number of cells exhibiting annexin V binding activity increased 6- and 5-fold, respectively (P < 0.05). A DNA microarray study showed significant (P < 0.05) upregulation of interleukin (IL)-1 β-convertase, and caspase-3, -8 and -9 genes in cHSP60- and cHSP10-stimulated than in control cells as confirmed by real-time RT-PCR and western blotting. Transcript levels of IL-1β and IL-18 in cells treated with cHSP60 and cHSP10 were found to be significantly (P < 0.05) higher in stimulated than in control cells.cHSP60- and cHSP10-induced caspase expression, proinflammatory cytokine production and apoptosis of primary cervical epithelial cells might play a role in the pathogenesis of infertility in women with persistent chlamydial infection.
Starting Page	69
Ending Page	78
Page Count	10
File Format	PDF
ISSN	10233830
Journal	Inflammation Research
Volume Number	60
Issue Number	1
e-ISSN	1420908X
Language	English
Publisher	SP Birkhäuser Verlag Basel
Publisher Date	2010-08-05
Publisher Place	Basel
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Chlamydia trachomatis cHSP60 cHSP10 Apoptosis Epithelial cells Neurology Dermatology Allergology Rheumatology Pharmacology/Toxicology Immunology
Content Type	Text
Resource Type	Article
Subject	Pharmacology Immunology

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