NDLI: Effects of dendritic polyglycerol sulfate on articular chondrocytes

Content Provider	Springer Nature Link
Author	Schneider, Tobias Welker, Pia Haag, Rainer Dernedde, Jens Hug, Thomas Licha, Kai Kohl, Benjamin Arens, Stephan Ertel, Wolfgang Schulze Tanzil, Gundula
Copyright Year	2015
Abstract	Inflammatory processes driven by cytokines play a crucial role during osteoarthritis (OA) progression. Dendritic polyglycerol sulfate (dPGS) was analyzed in vitro for its effects on articular chondrocytes, cartilage and cytokines involved in the OA process.The metabolic activity of cultured human articular chondrocytes stimulated for 24 h with dPGS (10−3–10−6 mol/L) was monitored using AlamarBlue® assay. The dPGS uptake was studied using fluorescence labeled nanoparticles. Further, chondrocytes were either treated with 10−6 M dPGS, TNFα (10 ng/mL) alone or with a combination of both. The influence on extracellular matrix components, pro- and anti-inflammatory cytokines, matrix metalloproteinase (MMP)1 and the anaphylatoxin receptor C3aR was analyzed by RTD-PCR, flow cytometry and ELISA.Even at higher dosages (10−3 mol/L), dPGS did not influence chondrocytes viability. Uptake of dPGS was successfully monitored in human articular chondrocytes and synovial fibroblasts, penetration into cartilage chips was up to ~50 µm. Cellular treatment with dPGS had no effect on synthesis of pro-inflammatory cytokines TNFα and IL-6, but expression of the anti-inflammatory IL-10 was upregulated. Cotreatment with TNFα and dPGS reduced the TNFα level, while IL-1β, IL-6 and IL-10 expression did not change. Collagen type II gene expression was significantly reduced after preincubating cells with dPGS, but remained unaffected at the protein level.Results indicate that dPGS could play a role in regulation of cytokines associated with the inflammatory aspect of OA progression.
Starting Page	917
Ending Page	928
Page Count	12
File Format	PDF
ISSN	10233830
Journal	Inflammation Research
Volume Number	64
Issue Number	11
e-ISSN	1420908X
Language	English
Publisher	Springer Basel
Publisher Date	2015-09-09
Publisher Place	Basel
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Chondrocytes Nanoparticles Synovial fibroblasts Cytokines Immunology Pharmacology/Toxicology Rheumatology Allergology Dermatology Neurology
Content Type	Text
Resource Type	Article
Subject	Pharmacology Immunology

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