NDLI: The lipopolysaccharide-induced pro-inflammatory response in RAW264.7 cells is attenuated by an unsaturated fatty acid–bovine serum albumin complex and enhanced by a saturated fatty acid

Content Provider	Springer Nature Link
Author	Chang, Chi Fen Chau, Yat Pang Kung, Hsiu Ni Lu, Kuo Shyan
Copyright Year	2011
Abstract	A 1:1 ratio of fatty acid (FA)–albumin complex was chosen to mimic physiological conditions, and the effects of FA–bovine serum albumin (BSA) complexes were tested in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells.Nitric oxide (NO) and various proteins/factors in RAW264.7 cells were quantified as follows: NO by the Griess assay; prostaglandin (PG) E2, interleukin (IL)-6 and tumor necrosis factor (TNF)-α by ELISA; inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 by Western blotting; and NF-κB and CD14/TLR4 by Western blotting or flow cytometry.BSA- or FA–BSA-treated RAW264.7 cells without LPS stimulation did not show any significant changes in NO or the tested proteins/factors and thus did not have any pro-inflammatory responses. Pre-treatment with unsaturated FA–BSA complexes significantly decreased the production of LPS-induced NO, PGE2, IL-6 and TNF-α, the expression of iNOS, COX-2 and CD14, IκB degradation and NF-κB translocation. On the contrary, pre-treatment with saturated FA–BSA complexes enhanced these LPS-induced pro-inflammatory factors and the subsequent responses.We concluded that unsaturated FA–BSA complexes, but not saturated FA–BSA complexes, exert an inhibitory effect on the LPS-induced pro-inflammatory response and that this effect may be partially mediated through suppression of the NF-κB signaling pathway. We suggest that an increase of unsaturated FA–BSA complexes may enhance the host’s defense against bacterial infection.
Starting Page	151
Ending Page	160
Page Count	10
File Format	PDF
ISSN	10233830
Journal	Inflammation Research
Volume Number	61
Issue Number	2
e-ISSN	1420908X
Language	English
Publisher	SP Birkhäuser Verlag Basel
Publisher Date	2011-11-18
Publisher Place	Basel
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Fatty acid–bovine serum albumin complex Inflammatory response Lipopolysaccharide Macrophage Cytokine Neurology Rheumatology Pharmacology/Toxicology Dermatology Allergology Immunology
Content Type	Text
Resource Type	Article
Subject	Pharmacology Immunology

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