NDLI: Involvement of the β 2-integrin CD18 in apoptosis signal transduction in human neutrophils

Content Provider	Springer Nature Link
Author	Larsson, J. Serrander, L. Stendahl, O. Lundqvist Gustafsson, H.
Copyright Year	2000
Abstract	Objective and design: To examine the hypothesis that an accelerated rate of neutrophil apoptosis occurs following β 2-integrin activation, and further investigate the signal transduction pathways involved.¶Material: Human polymorphonuclear neutrophils.¶Treatment: Neutrophils were challenged with pansorbins coated with antibodies towards the β 2-integrin subunit CD18 in a proportion of 1:100 with or without the inhibitors diphenylene iodonium (10 M), cytochalasin B (5 μg/ml), genistein (10 nM), herbimycin A (10 M) and Z-VAD-FMK (10 μM).¶Methods: Measurement of phosphatidylserine exposure and DNA fragmentation in flow cytometry and assessment of H2O2-production through spectrofluorometry. The results were analysed using Mann Whitney U test and Kruskal Wallis.¶Results: Pansorbins coated with antibodies to CD18 induce apoptosis in neutrophils (p<0.01), and activate the production of reactive oxygen species (p<0.01). Pre-treatment with the inhibitors have no effect on anti-CD18 induced apoptosis.¶Conclusion: Anti-CD18 pansorbins induce apoptosis in neutrophils through an alternative pathway not involving reactive oxygen species and independent of tyrosine phosphorylation, cytoskeletal reorganisation and caspases.¶
Starting Page	452
Ending Page	459
Page Count	8
File Format	PDF
ISSN	10233830
Journal	Inflammation Research
Volume Number	49
Issue Number	9
e-ISSN	1420908X
Language	English
Publisher	Birkhäuser-Verlag
Publisher Date	2014-02-04
Publisher Place	Basel
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Immunology Pharmacology/Toxicology Rheumatology Allergology Dermatology Neurology
Content Type	Text
Resource Type	Article
Subject	Pharmacology Immunology

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