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Identification of a B-cell antigenic epitope at the N-terminus of SARS-CoV M protein and characterization of monoclonal antibody against the protein
| Content Provider | Semantic Scholar |
|---|---|
| Author | Qian, Chao Qin, Di Tang, Qiao Zeng, Yi Tang, Guixia Lu, Chun |
| Copyright Year | 2006 |
| Abstract | To identify the potential B-cell antigenic epitopes within the N-terminus of SARS-CoV (SARS-associated coronavirus, SARS-CoV) M protein and characterize monoclonal antibody (MAb) against the protein as well as its recognizing region, we expressed and purified a portion of SARS-CoV M protein (amino acid 1–43) in Escherichia coli (E. coli). By using Western blot and enzyme-linked immunosorbent assay (ELISA), we showed that the purified recombinant M protein could be recognized by four SARS-CoV-positive human sera even when those sera were 12,800-fold diluted. Furthermore, we characterized one representative IgG2 MAb, 3H9, which exhibited a strong immunoreaction to both recombinant M protein and native viral protein of SARS-CoV. We found a B-cell antigenic epitope located between amino acid 1–15 and defined the MAb recognizing region within amino acid 16–28 of M. These findings not only suggest that both recombinant M protein and its specific MAbs may be used as the diagnostic reagents for SARS, but also provide a potential target site for the design of an epitope-based vaccine against SARS. |
| File Format | HTM / HTML |
| DOI | 10.1007/s11262-005-0050-8 |
| Journal | Virus Genes |
| PubMed Central reference number | PMC7088559 |
| PubMed reference number | 16972028 |
| Language | English |
| Publisher | PMC |
| Access Restriction | Open |
| Rights Holder | no-cc |
| Content Type | Text |
| Resource Type | Article Preprint |
