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Physiological properties of spinal lamina II GABAergic neurons in mice following peripheral nerve injury.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Schoffnegger, Doris Heinke, Bernhard Sommer, Claudia Sandkühler, Jürgen |
| Copyright Year | 2006 |
| Abstract | Aberrant GABAergic inhibition in spinal dorsal horn may underlie some forms of neuropathic pain. Potential, but yet unexplored, mechanisms include reduced excitability, abnormal discharge patterns or altered synaptic input of spinal GABAergic neurons. To test these hypotheses, we quantitatively compared active and passive membrane properties, firing patterns in response to depolarizing current steps and synaptic input of GABAergic neurons in spinal dorsal horn lamina II of neuropathic and of control animals. Transgenic mice were used which expressed enhanced green fluorescent protein (EGFP) controlled by the GAD67 promoter, thereby labelling one-third of all spinal GABAergic neurons. In all neuropathic mice included in this study, chronic constriction injury of one sciatic nerve led to tactile allodynia and thermal hyperalgesia. Control mice were sham-operated. Membrane excitability of GABAergic neurons from neuropathic or sham-treated animals was indistinguishable. The most frequent firing patterns observed in neuropathic and sham-operated animals were the initial burst (neuropathic: 46%, sham-treated: 42%), the gap (neuropathic: 31%, sham-treated: 29%) and the tonic firing pattern (neuropathic: 16%, sham-treated: 24%). The synaptic input from dorsal root afferents was similar in neuropathic and in control animals. Thus, a reduced membrane excitability, altered firing patterns or changes in synaptic input of this group of GABAergic neurons in lamina II of the spinal cord dorsal horn are unlikely causes for neuropathic pain. |
| Starting Page | 47 |
| Ending Page | 50 |
| Page Count | 4 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://cbr.meduniwien.ac.at/fileadmin/db_files/pub_art_29.pdf |
| Alternate Webpage(s) | https://neurocluster-db.meduniwien.ac.at/db_files/pub_art_29.pdf |
| PubMed reference number | 17053034v1 |
| Volume Number | 577 |
| Part | 3 |
| Journal | The Journal of physiology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Afferent neuron Dopaminergic Neurons External granular layer of cerebral cortex Ganglia, Spinal Green Fluorescent Proteins Hyperalgesia Mandibular right second molar tooth Neuralgia Peripheral Nerve Injuries Peripheral Nerves Structure of posterior gray horn of spinal cord Structure of sciatic nerve Substantia Gelatinosa Tactile Allodynia Tissue membrane enhanced green fluorescent protein nerve injury |
| Content Type | Text |
| Resource Type | Article |
