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A Genotype-independent Agrobacterium Mediated Transformation of Germinated Embryo of Cotton ( Gossypium Hirsutum L . )
| Content Provider | Semantic Scholar |
|---|---|
| Author | Kumar, Manoj Shukla, Anoop Kumar Singh, Harpal Verma, Praveen Chandra Singh, Kushal |
| Copyright Year | 2013 |
| Abstract | Explants transformation through Agrobacterium, followed by in vitro regeneration is only method used for genetic improvement of cotton. The currently used method are based on somatic embryogenesis, therefore time consuming, genotype-dependent and expensive. We report here a genotype-independent method for genetic transformation of cotton. Embryonic axes of 48 hours of germinating cotton seeds were incised at the cotyledonary node and co-cultivated with A. tumefaciens. Transfected embryos were transferred into test tubes and grown for 10 days at 28 + 2 o C. Later, the seedlings were transferred to pots in glass house. T1 seeds obtained from the putative T0 transgenics were screened for the promising transformation events on the basis of formation of root laterals in Hoagland liquid medium containing hygromycin B. The transgenic nature of the positive T1 seedlings was confirmed by PCR and PCR-Southern analysis. Histochemical assay showed the presence of the GUS reporter protein in T0 and T1 transgenics. This method gives 2-3 % transformation efficiency and provides convenient method for transformation of cotton. The strategy, in principle, should be applicable to all the cultivars and genotypes of cotton which are susceptible to Agrobacterium tumefaciens infection. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.tjprc.org/publishpapers/2-26-1362485025-9.A%20Genotype.full.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
