NDLI: Development of PCR Primers for Specific Identification and Detection of Botrytis cinerea on Tomato

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Development of PCR Primers for Specific Identification and Detection of Botrytis cinerea on Tomato

Content Provider	Semantic Scholar
Author	Song, Jeong Young Nam, Myeong Hyeon Kim, Byung-Sup
Copyright Year	2008
Abstract	Botrytis cinerea, gray mold pathogen, causes serious losses in greenhouse tomato crop. In this study, a primer set was developed for identification and specific PCR detection of B. cinerea from tomato plants. The primer pair (BTF1/BTR1) was designed from polymorphic sequence region in pyruvate carboxylase gene (pyc) of B. cinerea. A PCR product (112 bp) was amplified on genomic DNA of 13 B. cinerea isolates from 10 different host plants, but not on those from 6 other Botrytis spp., 4 Botryotinia spp., 5 Sclerotinia spp. and 16 other genus of phytopathogenic fungi. The sensitivity limit of the primer set was 2 pg of genomic DNA of B. cinerea, approximately. The PCR assay using species-specific primer set was specifically able to detect the pathogen on naturally infected tomato plants and artificially infected plants. These results suggest that the sensitivity and specificity of this primer set can be applied in a rapid and accurate diagnosis of tomato disease caused by B. cinerea.
Starting Page	138
Ending Page	143
Page Count	6
File Format	PDF HTM / HTML
DOI	10.4489/KJM.2008.36.2.138
Volume Number	36
Alternate Webpage(s)	http://ocean.kisti.re.kr/downfile/volume/mycology/GNHHDL/2008/v36n2/GNHHDL_2008_v36n2_138.pdf
Alternate Webpage(s)	https://doi.org/10.4489/KJM.2008.36.2.138
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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