NDLI: An SV40 “enhancer trap” incorporates exogenous enhancers or generates enhancers from its own sequences

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An SV40 “enhancer trap” incorporates exogenous enhancers or generates enhancers from its own sequences

Content Provider	Semantic Scholar
Author	Weber, Frank Gerd Villiers, Jean De Schaffner, Walter
Copyright Year	1984
Abstract	We have transfected monkey CV-1 cells with non-infectious, linear SV40 DNA, lacking the 72 bp repeat enhancer region. Infectious virus was recovered from this "enhancer trap" upon cotransfection with enhancer DNA segments from various viruses, notably a truncated polyoma enhancer that was integrated as a dimer. Cotransfection of the "enhancer trap" with fragmented DNA of mouse, monkey, or human origin yielded no recombinant virus with integrated cellular sequences, with one possible exception. In some transfection experiments without added viral enhancer DNA, SV40 variants were generated that have a segment of their flanking "late" DNA duplicated to substitute for the deleted 72 bp repeat. In one variant, an 88 bp duplication creates a strong enhancer from this nonenhancing DNA region. Both the polyoma enhancer fragment and the spontaneously created enhancers lack the alternating purines-pyrimidines or "CACA box" suggested to be characteristic for enhancer elements and show only limited homology to the "GTGG(AAATTT)G box."
Starting Page	983
Ending Page	992
Page Count	10
File Format	PDF HTM / HTML
DOI	10.1016/0092-8674(84)90048-5
PubMed reference number	6323032
Journal	Medline
Volume Number	36
Alternate Webpage(s)	https://api.elsevier.com/content/article/pii/0092867484900485
Alternate Webpage(s)	https://www.sciencedirect.com/science/article/pii/0092867484900485?dgcid=api_sd_search-api-endpoint
Alternate Webpage(s)	https://doi.org/10.1016/0092-8674%2884%2990048-5
Journal	Cell
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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