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Transdiferenciación de células mesenquimales del tejido adiposo: hacia una terapia celular para enfermedades degenerativas de la retina
| Content Provider | Semantic Scholar |
|---|---|
| Author | Marcos, S. Jaén, Ana Gonzalez, Deyarina Andreu, Stephen Avilés, Mora Fernández, Eduardo |
| Copyright Year | 2008 |
| Abstract | espanolObjetivos: Obtencion de celulas troncales mesenquimales de tejido adiposo para trasplante y recuperacion funcional de retinas degeneradas. Material y metodologia: Extraccion de la grasa inguinal de ratones transgenicos para proteina verde fluorescente y aislamiento de las celulas troncales mesenquimales. Estas celulas fueron implantadas utilizando inoculaciones intravitreas y subretinianas en un modelo murino (rd10) que asemeja la retinosis pigmentaria autosomal recesiva humana. Expresion de genes involucrados en el desarrollo de la retina mediante RT-PCR. Resultados: Nuestros resultados demuestran que en ambos abordajes quirurgicos las celulas trasplantadas sobreviven, migran y se integran en diferentes capas de la retina de los ratones rd10. Ademas hemos identificado la expresion de genes en embriones y cuerpos embrionarios (Otx2, NF200,Pax6 periferina, IRBP) que pueden ser utilizados como activadores o potenciadores de la transdiferenciacion. Conclusiones: las celulas mesenquimales del tejido adiposo reunen las condiciones necesarias para su potencial uso en la terapia de enfermedades degenerativas de la retina. Los cuerpos embrionarios representan un modelo util para estudiar los mecanismos reguladores de la diferenciacion de las celulas retinianas. EnglishObjectives: To obtain adult mesenchymal stem cells from adipose tissue to be used in cellular therapies aimed to retinal regeneration. Material and Methods: isolation of adult mesenchymal stem cells from adipose tissue of green fluorescent protein transgenic mice. Recipient mouse eyes were transplanted with these cells by using intravitreal and subretinal procedures. We have used a mice model of human autosomal recessive retinitis pigmentosa (rd10). Gene expression by means of RT-PCR of mRNA isolated from embryo eyes (E9,5 to E18,5); post-natal eyes (P1 to P10) and adults eyes .That technique was also used in mouse embryoid bodies spontaneously differentiated from embryonic stem cells ES-D3. Results: In both surgical procedures we observed survival, migration and integration of mesenchymal stem cells into several retinal layers. We have also identified the expression of some retinal genes (Otx2, NF200,Pax6, peripherin,IRBP) wich may help to drive the mesenchymal stem cells transdifferentiation. Conclusions: Mesenchimal cells are very promising to be used in degenerative retinal diseases. We emphasize the usefulness of mouse embryoid bodies as a model to study the regulatory mechanisms of retinal neurons differentiation and its potential for obtaining retinal progenitors. |
| Starting Page | 183 |
| Ending Page | 190 |
| Page Count | 8 |
| File Format | PDF HTM / HTML |
| Volume Number | 19 |
| Alternate Webpage(s) | http://www.mapfre.com/fundacion/html/revistas/trauma/v19n3/pdf/02_08.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
