The Validation of an LC-MS Method for the Determination of Risperidone and its Active Metabolite 9-Hydroxyrisperidone in Human Plasma

Content Provider	Semantic Scholar
Author	Zhang, Li Jiao, Zheng Kuan Yao, Zouqing Zhong, Yan Jun Zhong, Mingkang Yu, Yunqiu
Copyright Year	2005
Abstract	A simple, specific and sensitive high performance liquid chromatography-mass spectrometry (LC-MS) method for the determination of risperidone and its active metabolite 9-hydroxyrisperidone in human plasma has been developed and validated. The analytes were prepared through a single-step liquid-liquid extraction (LLE) procedure with the solvent methyl tert-butyl ether and quantitated by MS detection in the positive mode using selected ion monitoring (SIM). Each analytical run was completed within 9 min. Results showed that the LC-MS method enabled to detection of both compounds down to 0.1 ng.mL−1 (S/N > 3) and the linear range was 0.2–24 ng.mL−1, with the correlation coefficients above 0.99. At the concentration of 0.2, 0.5, 10 and 20 ng.mL−1, the inter-day and intra-day RSD were both below 15%. The method has been successfully used to support the routine therapeutic drug monitoring (TDM) and the pharmacokinetics study of risperidone.
Starting Page	245
Ending Page	251
Page Count	7
File Format	PDF HTM / HTML
DOI	10.1365/s10337-005-0506-y
Volume Number	61
Alternate Webpage(s)	https://page-one.springer.com/pdf/preview/10.1365/s10337-005-0506-y
Alternate Webpage(s)	https://doi.org/10.1365/s10337-005-0506-y
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article