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Two alkaline phosphatase genes are expressed during early development in the mouse embryo.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Hahnel, Ann C. Rappolee, Daniel A. Millán, José Luis Manes, T. Ziomek, Carol Ann Theodosiou, N. G. Werb, Zena Pedersen, Roger A. Schultz, Gilbert A. |
| Copyright Year | 1990 |
| Abstract | Alkaline phosphatase (AP) activity is stage specific in mouse embryos and may be associated with compaction and separation of trophectoderm from inner cell mass in preimplantation development. We previously sequenced a cDNA and two mouse AP genes that could contribute to the AP activity in embryos. Oligonucleotide primers were constructed from the three sequences and used in the reverse transcription-polymerase chain reaction technique to establish that two of the three AP isozymes are transcribed during preimplantation development. The predominant transcript (E-AP) is from a gene highly homologous to the human tissue-specific APs, but different from the mouse intestinal AP. Tissue non-specific (TN) AP also is transcribed, but there is approximately 10 times less TN-AP than E-AP transcript. The TN-AP isozyme is the predominant transcript of 7 to 14 day embryos and primordial germ cells. A switch in predominance from E-AP to TN-AP must occur during early postimplantation development. This study establishes a framework for experiments to determine the functions of the two isozymes during preimplantation development. |
| File Format | PDF HTM / HTML |
| PubMed reference number | 2133555 |
| Journal | Medline |
| Volume Number | 110 |
| Issue Number | 2 |
| Alternate Webpage(s) | http://dev.biologists.org/content/develop/110/2/555.full.pdf |
| Alternate Webpage(s) | http://digitalcommons.wayne.edu/cgi/viewcontent.cgi?article=1001&context=med_obgyn |
| Journal | Development |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
