NDLI: Cryopreservation of transgenic Huntington's disease rhesus macaque sperm-A Case Report.

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Cryopreservation of transgenic Huntington's disease rhesus macaque sperm-A Case Report.

Content Provider	Semantic Scholar
Author	Putkhao, Kittiphong Chan, Anthony W. S. Agca, Yuksel Parnpai, Rangsun
Copyright Year	2013
Abstract	The cryoprotective effects of glycerol in three different semen freezing extenders, Tris-citrate (TRIS), TEST, and Tes-Tris-Egg yolk (TTE), on wild-type (WT) rhesus monkey (Macaca mulatta) sperm cryopreservation have been tested. Sperm motility and viability were examined to evaluate the integrity of frozen-thawed sperm, and the best extender was selected to cryopreserve sperm from transgenic Huntington's disease (HD) rhesus monkey. The results showed no post-thaw motility difference among the freezing extender tested (P>0.05). However, sperm membrane integrity in TEST and TTE were significantly better than in TRIS extender (P<0.05). TEST was chosen for HD rhesus monkey sperm cryopreservation. The results showed that post-thawed HD sperm motility and viability was not different compared with WT control group (P>0.05). The present study demonstrates that TEST and TTE were excellent extenders and suitable for rhesus monkey sperm cryopreservation and no detectible differences of post-thaw sperm motility and viability between HD and WT rhesus monkeys resulted from TEST extender.
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://open.library.emory.edu/publications/emory:mr96w/pdf/
Alternate Webpage(s)	https://www.omicsgroup.org/pdfdownload.php?aid=21341&download=journals/cryopreservation-of-transgenic-huntingtons-disease-rhesus-macaque-sperm-a-case-report-2168-9849.1000116.pdf
Alternate Webpage(s)	https://www.omicsonline.org/open-access/cryopreservation-of-transgenic-huntingtons-disease-rhesus-macaque-sperm-a-case-report-2168-9849.1000116.pdf
PubMed reference number	25431746v1
Volume Number	2
Journal	Cloning & transgenesis
Language	English
Access Restriction	Open
Subject Keyword	Cryopreservation Egg Food Product Extender Device Component Freezing Glycerin Huntington Disease Mamu-B 01 antigen, Macaca mulatta Sperm Motility Spermatozoa Cell Count Tissue membrane Transthoracic echocardiography Tromethamine cryopreservation; sperm sodium citrate sperm cell yolk
Content Type	Text
Resource Type	Report

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