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Molecular cloning of a ribosomal protein gene from the fission yeast Schizosaccharomyces pombe
| Content Provider | Semantic Scholar |
|---|---|
| Author | Nischt, Roswitha Thüroff, Eduardo Käufer, Norbert F. |
| Copyright Year | 2004 |
| Abstract | SummaryUsing the structural gene for the ribosomal protein L3 from Saccharomyces cerevisiae as a probe, we isolated a homologous fragment from genomic DNA of Schizosaccharomyces pombe. Analysis of the plasmid carrying this fragment by hybridization selection and 2D-electrophoresis revealed a 31 kDa ribosomal protein. Transformation of the vector pDB248x containing this fragment into Schizosaccharomyces pombe leads to an increased level of mRNA suggesting that we have cloned the entire and actively transcribed gene. |
| Starting Page | 365 |
| Ending Page | 370 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| DOI | 10.1007/BF00418408 |
| PubMed reference number | 3442819 |
| Journal | Medline |
| Volume Number | 10 |
| Alternate Webpage(s) | https://page-one.springer.com/pdf/preview/10.1007/BF00418408 |
| Alternate Webpage(s) | https://doi.org/10.1007/BF00418408 |
| Journal | Current Genetics |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |