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Mitotic regulation of the human anaphase-promoting complex by phosphorylation.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Kraft, Claudine Herzog, Franz Gieffers, Christian Mechtler, Karl Hagting, Anja Pines, Jonathon Peters, J. G. |
| Copyright Year | 2003 |
| Abstract | The anaphase-promoting complex (APC) or cyclosome is a ubiquitin ligase that initiates anaphase and mitotic exit. APC activation is thought to depend on APC phosphorylation and Cdc20 binding. We have identified 43 phospho-sites on APC of which at least 34 are mitosis specific. Of these, 32 sites are clustered in parts of Apc1 and the tetratricopeptide repeat (TPR) subunits Cdc27, Cdc16, Cdc23 and Apc7. In vitro, at least 15 of the mitotic phospho-sites can be generated by cyclin-dependent kinase 1 (Cdk1), and 3 by Polo-like kinase 1 (Plk1). APC phosphorylation by Cdk1, but not by Plk1, is sufficient for increased Cdc20 binding and APC activation. Immunofluorescence microscopy using phospho-antibodies indicates that APC phosphorylation is initiated in prophase during nuclear uptake of cyclin B1. In prometaphase phospho-APC accumulates on centrosomes where cyclin B ubiquitination is initiated, appears throughout the cytosol and disappears during mitotic exit. Plk1 depletion neither prevents APC phosphorylation nor cyclin A destruction in vivo. These observations imply that APC activation is initiated by Cdk1 already in the nuclei of late prophase cells. |
| Starting Page | 74 |
| Ending Page | 80 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.bio.brandeis.edu/classes/biol200/mitosis%20papers/5Peters%20APC%20Cdk1%2003.pdf |
| PubMed reference number | 14657031v1 |
| Volume Number | 22 |
| Issue Number | 24 |
| Journal | The EMBO journal |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | CDC16 gene CDK1 gene CDK6-associated protein p18 Centrosome Cyclin B Cyclin-Dependent Kinases Cyclins Cytoplasmic matrix Fluorescent Antibody Technique Ligase Mitotic Prophase Prometaphase Promotion (action) SLC25A24 gene Trinucleotide Repeat Expansion anaphase-promoting complex location eIF-2 Kinase exit from mitosis |
| Content Type | Text |
| Resource Type | Article |
