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Detection and characterization of hepatitis C virus RNA in seminal plasma and spermatozoon fractions of semen from patients attempting medically assisted conception.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Bourlet, Thomas Levy, Rachel Maertens, Anne Tardy, Jean Claude Grattard, Florence Cordonier, Hélène Laurent, Jean-Louis Guérin, J. -P. Pozzetto, Bruno |
| Copyright Year | 2002 |
| Abstract | To investigate the risk of transmission of hepatitis C virus (HCV) via semen in assisted reproduction techniques, semen samples from 32 men chronically infected with HCV attending a center for assisted procreation were tested for HCV RNA by a reverse transcription-PCR protocol by using a modified version of the Cobas AMPLICOR HCV assay (version 2.0; Roche Diagnostics). The sensitivity of the test was 40 copies/ml. Four of 32 seminal plasma samples (12.5%) were found to be positive for the presence of HCV RNA. The median HCV load in blood was significantly higher in patients who were found to be positive for the presence of HCV RNA in semen than in those who tested negative (P = 0.02). In one man, seven consecutive seminal plasma samples tested positive for HCV RNA, as did two consecutive motile spermatozoon fractions; the corresponding fractions obtained after migration of the spermatozoa remained negative. Despite the absence of the proven infectivity of virus in semen samples that test positive for HCV RNA, these findings highlight the fact that seminal fluid may exhibit prolonged HCV RNA excretion. The usefulness of HCV RNA detection in both seminal plasma and spermatozoon fractions before the start of a program of medically assisted reproduction in couples in whom the male partner is chronically infected with HCV would need to be evaluated prospectively with a larger population of subjects exhibiting HCV RNA in their semen. |
| Starting Page | 1680 |
| Ending Page | 1684 |
| Page Count | 5 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://jcm.asm.org/content/40/9/3252.full.pdf |
| PubMed reference number | 12202561v1 |
| Volume Number | 40 |
| Issue Number | 9 |
| Journal | Journal of clinical microbiology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Biotine Roche Chemical and Drug Induced Liver Injury Copy (object) Exhibits as Topic HCV viral load Hepatitis B Hepatitis C virus Hepatitis C, Chronic Large Patients RNA RNA, Viral Reproduction Reproductive Techniques Reverse Transcription Seminal fluid sperm cell |
| Content Type | Text |
| Resource Type | Article |
