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Detection of adsorption of Ru(II) and Os(II) polypyridyl complexes on gold and silver nanoparticles by single-photon counting emission measurements.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Glomm, Wilhelm Robert Moses, Selina Brennaman, Matthew K. Papanikolas, John M. Franzen, Stefan |
| Copyright Year | 2005 |
| Abstract | The present study describes a new application of ruthenium(II) tris(bipyridine) (Ru(bpy)3(2+)) and osmium(II) tris(bipyridine) (Os(bpy)3(2+)) as phosphorescent labels for the quantification of surface binding of molecules to gold and silver nanoparticles. The fraction of Ru(bpy)3(2+) and Os(bpy)3(2+) that is in solution can be distinguished from the surface-bound fraction by the relative lifetimes and integrated emission yields as determined by time-correlated single-photon counting (TCSPC) spectroscopy. Complementary steady-state measurements were carried out to confirm surface attachment of the phosphorescent label molecules. Although the emission of solutions of Ru(bpy)3(2+) and Os(bpy)3(2+) is quenched proportional to the concentration of 10 nm Au or 20 nm Ag nanoparticles, the quenching is static and not diffusional quenching observed in Stern-Volmer plots. The results demonstrate that time-resolved spectroscopy provides a rapid method for the measurement of surface binding of labeled molecules on metallic nanoparticles. While steady-state measurements require the preparation of a series of samples with varying quencher concentrations and a reference, the method described herein requires a single sample plus reference. The mechanism for phosphorescence quenching on Au and Ag nanoparticles is discussed in terms of energy and electron transfer theories. |
| File Format | PDF HTM / HTML |
| DOI | 10.1021/jp049184k |
| Alternate Webpage(s) | http://www4.ncsu.edu/~franzen/public_html/papers/JPC_B_109_804_2005.pdf |
| PubMed reference number | 16866445 |
| Alternate Webpage(s) | https://doi.org/10.1021/jp049184k |
| Journal | Medline |
| Volume Number | 109 |
| Issue Number | 2 |
| Journal | The journal of physical chemistry. B |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
