NDLI: Isolation of Feline Immunodeficiency Virus from Domestic Cats

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Isolation of Feline Immunodeficiency Virus from Domestic Cats

Content Provider	Semantic Scholar
Author	Masuda, M.
Copyright Year	1990
Abstract	63 Attempts were made to isolate feline immunodeficiency virus (FIV) from 23 domestic cats showing various clinical signs. T -lymphotropic viruses with Mg + + -dependent reverse transcriptase (RT) were isolated from 2 cats with chronic stoma titis. Virus isolation was achieved by cocultivation of peripheral blood lymphocytes (PBL) from the cats that were positive for feline leukemia virus (FeL V) p27 antighn by ELISA, with concanavalin A-stimulawed PBL from normal cats. A cytopathic effect (CPE) consisting of ballooning degeneration and giant cell formation was noted in the cell cultures within 3-10 days of cocultivation. Cytopathic changes were associated with the appearance of Mg + + -dependent RT in the culture fluids. The cell cultures showing CPE and RT activity were negative for FeL V p27 antigen. Electron micros copy of the RT -positive cultures revealed virus particles of morphologically typical lentivirus. Blood from one of the cats from which the viruses were isolated was ex perimentally inoculated into 2 normal cats. Both inoculated cats were positive for FeL V p27 antigen 21 days after the inoculation, showed immunodeficiency-like syn drome, and died 70 days after the inoculation. FIV -like viruses were also isolated from these cats by co cultivation of PBL and the cultures were negative for FeLV antigen. Experimental infection with FIV or FeLV alone has not caused immuno deciciency-like syndrome. These findings suggest that FeLV may play an important role as a cofactor for the exhibition of immunodeficiency syndrome.
Starting Page	63
Ending Page	63
Page Count	1
File Format	PDF HTM / HTML
Volume Number	38
Alternate Webpage(s)	https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/3210/1/KJ00002377363.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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