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Determination of 13 amino acids in Asini Corii Colla by RP-HPLC with precolumn derivatization
| Content Provider | Semantic Scholar |
|---|---|
| Author | Ping-Hong, Chen Shu-Fang, Wang Xingchu, Gong |
| Copyright Year | 2013 |
| Abstract | Objective To establish a RP-HPLC method for the simultaneous determination of 13 amino acids in Asini Corii Colla. Methods Asini Corii Colla was hydrolyzed for 3 h at 120 ℃ using 6 mol/L hydrochloric acid, and the amino acids were determined by RP-HPLC after being derived with phenyl isothiocyanate. HPLC was performed on an Eclipse AAA column (75 mm × 4.6 mm, 3.5 μm) with acetonitrile-110 mmol/L ammonium acetate solution (1 : 99) as mobile phase A and acetic acid-acetonitrile-water (0.05 : 80 : 20) as mobile phase B in the gradient mode at a flow rate of 0.6 mL/min. The column temperature was 42 , ℃ and the detection wavelength was set at 254 nm. Results The 13 amino acids were separated within 36 min with a good linearity (r > 0.9977) in the range of the test concentration. The average recoveries (n = 3) were 95.2%—104.4% and the RSD values were less than 5.0%. Conclusion The method is simple, accurate, and repeatable, which could be available for the quality control of Asini Corii Colla. It may also serve as a good reference for the determination of amino acids in other pharmaceuticals. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.tiprpress.com/zcy/ch/reader/create_pdf.aspx?falg=1&file_no=201314024&quarter_id=14&year_id=2013 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
