NDLI: Superoxide anion formation from lucigenin: an electron spin resonance spin-trapping study.

Content Provider	Semantic Scholar
Author	Vásquez-Vivar, Jeannette Hogg, N. Pritchard, Kirkwood A. Martasek, P. Kalyanaraman, Balaraman
Copyright Year	1997
Abstract	Lucigenin (LC2+) is frequently used as a superoxide probe. To detect superoxide, lucigenin must be reduced to the lucigenin cation radical (LC.+). We show, using the phosphorylated spin trap 5-diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide (DEPMPO), that lucigenin stimulates NADPH-dependent superoxide production by endothelial nitric oxide synthase (eNOS). The formation of the DEPMPO-superoxide adduct is calcium/calmodulin independent. DEPMPO-superoxide adduct formation is inhibited by diphenyleneiodonium and is abolished by superoxide dismutase. It is likely that eNOS/NADPH can reduce lucigenin to LC.+ which reduces oxygen to superoxide. Consequently, lucigenin cannot be used to measure superoxide formation.
File Format	PDF HTM / HTML
DOI	10.1016/S0014-5793(97)00036-7
PubMed reference number	9042951
Journal	Medline
Volume Number	403
Issue Number	2
Alternate Webpage(s)	https://core.ac.uk/download/pdf/82189762.pdf
Alternate Webpage(s)	https://doi.org/10.1016/S0014-5793%2897%2900036-7
Journal	FEBS letters
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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