NDLI: Multicomponent polysaccharide–protein bioconjugation in the development of antibacterial glycoconjugate vaccine candidates† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c7sc05467j

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Multicomponent polysaccharide–protein bioconjugation in the development of antibacterial glycoconjugate vaccine candidates† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c7sc05467j

Content Provider	Semantic Scholar
Author	Méndez, Yanira González Chang, Janoi Humpierre, Ana R. Zanuy, Abel Garrido, Raine Vasco, Aldrin V. Pedroso, Jessy Santana, Darielys Rodríguez, Laura Martín García-Rivera, Dagmar Valdés, Yury Vérez-Bencomo, Vicente Rivera, Daniel G.
Copyright Year	2018
Abstract	A new synthetic strategy for the development of multivalent antibacterial glycoconjugate vaccines is described. The approach comprises the utilization of an isocyanide-based multicomponent process for the conjugation of functionalized capsular polysaccharides of S. pneumoniae and S. Typhi to carrier proteins such as diphtheria and tetanus toxoids. For the first time, oxo- and carboxylic acid-functionalized polysaccharides could be either independently or simultaneously conjugated to immunogenic proteins by means of the Ugi-multicomponent reaction, thus leading to mono- or multivalent unimolecular glycoconjugates as vaccine candidates. Despite the high molecular weight of the two or three reacting biomolecules, the multicomponent bioconjugation proved highly efficient and reproducible. The Ugi-derived glycoconjugates showed notable antigenicity and elicited good titers of functional specific antibodies. To our knowledge, this is the only bioconjugation method that enables the incorporation of two different polysaccharidic antigens to a carrier protein in a single step. Applications in the field of self-adjuvanting, eventually anticancer, multicomponent vaccines are foreseeable.
Starting Page	2581
Ending Page	2588
Page Count	8
File Format	PDF HTM / HTML
DOI	10.1039/c7sc05467j
Alternate Webpage(s)	http://pubs.rsc.org/en/content/articlepdf/2018/sc/c7sc05467j
Alternate Webpage(s)	http://www.rsc.org/suppdata/c7/sc/c7sc05467j/c7sc05467j1.pdf
PubMed reference number	29719713
Alternate Webpage(s)	https://doi.org/10.1039/c7sc05467j
Journal	Medline
Volume Number	9
Journal	Chemical science
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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