Expression of lectin-like oxidized low-density lipoprotein receptors during ischemia-reperfusion and its role in determination of apoptosis and left ventricular dysfunction.

Content Provider	Semantic Scholar
Author	Li, Dayuan Williams, Victor Liu, Ling Chen, Hongjiang Sawamura, Tatsuya Romeo, Francesco Mehta, Jawahar Lal
Copyright Year	2003
Abstract	OBJECTIVES The goal of this study was to determine the role of lectin-like oxidized low-density lipoprotein receptors (LOX-1), a recently identified oxidized low-density lipoprotein (ox-LDL) receptor, in ischemia-reperfusion injury to the heart. BACKGROUND Reactive oxygen species (ROS) released during ischemia-reperfusion oxidize low-density lipoproteins; LOX-1 is upregulated by ox-LDL and ROS, and is involved in cell injury. METHODS Anesthetized rats were subjected to left coronary artery ligation for 60 min (n = 10, ischemia group), or ischemia followed by 60 min of reperfusion (n = 30, ischemia-reperfusion group). Rats in the latter group were treated with saline, the LOX-1 blocking antibody JXT21 (10 mg/kg), or nonspecific anti-goat immunoglobulin G (IgG) (10 mg/kg). Ten other rats underwent thoracotomy without coronary ligation (sham control). RESULTS Ischemia-reperfusion was associated with an increase in LOX-1 expression, lipid peroxidation and apoptosis, a large infarct area, and a decrease in left ventricular function (all, p < 0.01 vs. sham control and ischemia alone groups). Treatment of rats with LOX-1 antibody prevented ischemia-reperfusion-induced upregulation of LOX-1. Importantly, the LOX-1 antibody reduced apoptosis by 48%, lipid peroxidation by 39%, and myocardial infarct size by 45%, and improved left ventricular function (first derivative of pressure measured over time: -47% to -18%, p < 0.01). Nonspecific IgG had no effect. CONCLUSIONS Lectin-like oxidized low-density lipoprotein receptors are upregulated during myocardial ischemia-reperfusion, and appear to be associated with apoptosis, necrosis, and left ventricular functional deterioration.
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Alternate Webpage(s)	http://www.onlinejacc.org/content/accj/41/6/1048.full.pdf?download=true
PubMed reference number	12651056v1
Volume Number	41
Issue Number	6
Journal	Journal of the American College of Cardiology
Language	English
Access Restriction	Open
Subject Keyword	Apoptosis Email Heart Diseases Immunotoxins Lectin Left Ventricular Function Left coronary artery structure Ligation Lipid Peroxidation Lipoprotein Receptor Low-Density Lipoproteins Myocardial Infarction Myocardial Ischemia Necrosis OLR1 gene Reactive Oxygen Species Reperfusion Injury Reperfusion Therapy Science Thoracotomy Up-Regulation (Physiology) Ventricular Dysfunction Ventricular Dysfunction, Left
Content Type	Text
Resource Type	Article