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Nociceptin Induces Hypophagia in the Perifornical and Lateral Hypothalamic Area
| Content Provider | Semantic Scholar |
|---|---|
| Author | Parsons, Matthew P. Burt, Julia Cranford, Amanda Alberto, Christian O. Zipperlen, Katrin Hirasawa, Michiru |
| Copyright Year | 2012 |
| Abstract | Nociceptin/orphanin FQ (N/OFQ) is known to induce food intake when administered into the lateral ventricle or certain brain areas. This is somewhat contradictory to its reward-suppressing role, as food is a strong rewarding stimulus. This discrepancy may be due to the functional diversity of N/OFQ's target brain areas. N/OFQ has been shown to inhibit orexin and melanin-concentrating hormone (MCH) neurons, both of which are appetite-inducing cells. As the expression of these neurons is largely confined to the lateral hypothalamus/perifornical area (LH/PFA), we hypothesized that N/OFQ inhibits food intake by acting in this area. To test this hypothesis, we examined the effect of local N/OFQ infusion within the LH/PFA on food intake in the rat and found that N/OFQ decreased sugar pellet as well as chow intake. This effect was not seen when the injection site was outside of the LH/PFA, suggesting a site-specific effect. Next, to determine a possible cellular mechanism of N/OFQ action on food intake, whole cell patch clamp recordings were performed on rat orexin neurons. As previously reported in mice, N/OFQ induced a strong and long lasting hyperpolarization. Pharmacological study indicated that N/OFQ directly inhibited orexin neurons by activating ATP-sensitive potassium (KATP) channels. This effect was partially but significantly attenuated by the inhibitors of PI3K, PKC and PKA, suggesting that the N/OFQ signaling is mediated by these protein kinases. In summary, our results demonstrate a KATP channel-dependent N/OFQ signaling and that N/OFQ is a site-specific anorexic peptide. |
| File Format | PDF HTM / HTML |
| DOI | 10.1371/journal.pone.0045350 |
| PubMed reference number | 23028954 |
| Journal | Medline |
| Volume Number | 7 |
| Alternate Webpage(s) | http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0045350&type=printable |
| Alternate Webpage(s) | http://research.library.mun.ca/609/3/nociceptin_induces_hypophagia.pdf |
| Alternate Webpage(s) | http://research.library.mun.ca/609/1/nociceptin_induces_hypophagia.pdf |
| Alternate Webpage(s) | https://doi.org/10.1371/journal.pone.0045350 |
| Journal | PloS one |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
