Chicken “erythroid” cells transformed by the Gag-Myb-Ets-encoding E26 leukemia virus are multipotent

Content Provider	Semantic Scholar
Author	Graf, Thomas McNagny, Kelly M. Brady, Gérard Frampton, Jonathan
Copyright Year	1992
Abstract	The E26 avian leukemia virus encodes a transcriptional activator-type oncoprotein consisting of Gag, Myb, and Ets domains, and transforms early erythroid cells as well as myeloblasts. Surprisingly, we have found that "early erythroid" transformants obtained in culture are multipotent, since they can be induced to differentiate into myeloblasts and eosinophils after superinfection with retroviruses containing kinase-type or ras oncogenes. In addition, TPA is an efficient inducer that generates predominantly eosinophils at low concentrations and myeloblasts at high concentrations. The determination process involves the complete extinction of erythroid/thrombocytic markers and the subsequent activation of myelomonocytic/eosinophilic properties, including the acquisition of specific growth factor requirements. "Erythroleukemic" cells from virus-infected animals were likewise found to be multipotent, making this a unique system to study the genesis of stem cell leukemias and the molecular basis of lineage commitment during hematopoiesis.
Starting Page	201
Ending Page	213
Page Count	13
File Format	PDF HTM / HTML
DOI	10.1016/0092-8674(92)90096-U
Alternate Webpage(s)	https://api.elsevier.com/content/article/pii/009286749290096U
Alternate Webpage(s)	https://www.sciencedirect.com/science/article/pii/009286749290096U?dgcid=api_sd_search-api-endpoint
PubMed reference number	1322247
Alternate Webpage(s)	https://doi.org/10.1016/0092-8674%2892%2990096-U
Journal	Medline
Volume Number	70
Journal	Cell
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article