Staphylococcus aureus mutants lacking the LytR-CpsA-Psr family of enzymes release cell wall teichoic acids into the extracellular medium.

Content Provider	Semantic Scholar
Author	Chan, Yvonne Gar-Yun Frankel, Matthew B. Dengler, Vanina Schneewind, Olaf Missiakas, Dominique
Copyright Year	2013
Abstract	The LytR-CpsA-Psr (LCP) proteins are thought to transfer bactoprenol-linked biosynthetic intermediates of wall teichoic acid (WTA) to the peptidoglycan of Gram-positive bacteria. In Bacillus subtilis, mutants lacking all three LCP enzymes do not deposit WTA in the envelope, while Staphylococcus aureus Δlcp mutants display impaired growth and reduced levels of envelope phosphate. We show here that the S. aureus Δlcp mutant synthesized WTA yet released ribitol phosphate polymers into the extracellular medium. Further, Δlcp mutant staphylococci no longer restricted the deposition of LysM-type murein hydrolases to cell division sites, which was associated with defects in cell shape and increased autolysis. Mutations in S. aureus WTA synthesis genes (tagB, tarF, or tarJ2) inhibit growth, which is attributed to the depletion of bactoprenol, an essential component of peptidoglycan synthesis (lipid II). The growth defect of S. aureus tagB and tarFJ mutants was alleviated by inhibition of WTA synthesis with tunicamycin, whereas the growth defect of the Δlcp mutant was not relieved by tunicamycin treatment or by mutation of tagO, whose product catalyzes the first committed step of WTA synthesis. Further, sortase A-mediated anchoring of proteins to peptidoglycan, which also involves bactoprenol and lipid II, was not impaired in the Δlcp mutant. We propose a model whereby the S. aureus Δlcp mutant, defective in tethering WTA to the cell wall, cleaves WTA synthesis intermediates, releasing ribitol phosphate into the medium and recycling bactoprenol for peptidoglycan synthesis.
Starting Page	224
Ending Page	237
Page Count	14
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://jb.asm.org/content/195/20/4650.full.pdf
PubMed reference number	23935043v1
Alternate Webpage(s)	https://doi.org/10.1128/JB.00544-13
DOI	10.1128/jb.00544-13
Journal	Journal of bacteriology
Volume Number	195
Issue Number	20
Language	English
Access Restriction	Open
Subject Keyword	Anabolism Autolysis Cell Wall Cell division Gram-Positive Bacteria Hydrolase KLHDC2 gene Mutation Periodontal screening and recording special finding:Find:Pt:Gingiva:Nom:PSR scale Recycling Ribitol Staphylococcus aureus enterotoxin B Ab.IgE:ACnc:Pt:Ser:Qn Teichoic Acids Thioctic Acid Tunicamycin bactoprenol gram inorganic phosphate mutant peptidoglycan biosynthetic process peptidoglycan location
Content Type	Text
Resource Type	Article