Analysis of Paper-Based Colorimetric Assays With a Smartphone Spectrometer

Content Provider	Semantic Scholar
Author	Woodburn, Elizabeth V. Long, Kenneth D. Cunningham, Brian T.
Copyright Year	2019
Abstract	We report on the adaptation of a smartphone’s rear-facing camera to function as a spectrometer that measures the spectrum of light scattered by common paper-based assay test strips. We utilize a cartridge that enables a linear series of test pads in a single strip to be swiped past the read head of the instrument while the phone’s camera records video. The strip is housed in a custom-fabricated cartridge that slides through the instrument to facilitate illumination with white light from the smartphone’s flash LED that is directed through an optical fiber. We demonstrate the ability to detect subtle changes in the scattered spectrum that enables quantitative analysis of single-analyte and multi-analyte strips. The demonstrated capability can be applied to broad classes of paper-based assays in which visual observation of colored strips is not sufficiently quantitative, and for which analysis of red-green-blue pixel values of a camera image are not capable of measuring complex scattered spectra.
Starting Page	508
Ending Page	514
Page Count	7
File Format	PDF HTM / HTML
DOI	10.1109/JSEN.2018.2876631
PubMed reference number	31579394
Journal	Medline
Volume Number	19
Alternate Webpage(s)	https://nano.ece.illinois.edu/files/2019/05/JSenSmartphoneSpectrometer2019.pdf
Alternate Webpage(s)	https://doi.org/10.1109/JSEN.2018.2876631
Journal	IEEE Sensors Journal
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article