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Calcium stiffens archaeal Rad51 recombinase from Methanococcus voltae for homologous recombination.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Qian, Xinguo He, Yujiong Ma, Xinfeng Fodje, Michel N. Grochulski, Pawel Luo, Yu Bing |
| Copyright Year | 2006 |
| Abstract | Archaeal RadA or Rad51 recombinases are close homologues of eukaryal Rad51 and DMC1. These and bacterial RecA orthologues play a key role in DNA repair by forming helical nucleoprotein filaments in which a hallmark strand exchange reaction between homologous DNA substrates occurs. Recent studies have discovered the stimulatory role by calcium on human and yeast recombinases. Here we report that the strand exchange activity but not the ATPase activity of an archaeal RadA/Rad51 recombinase from Methanococcus voltae (MvRadA) is also subject to calcium stimulation. Crystallized MvRadA filaments in the presence of CaCl(2) resemble that of the recently reported ATPase active form in the presence of an activating dose of KCl. At the ATPase center, one Ca(2+) ion takes the place of two K(+) ions in the K(+)-bound form. The terminal phosphate of the nonhydrolyzable ATP analogue is in a staggered conformation in the Ca(2+)-bound form. In comparison, an eclipsed conformation was seen in the K(+)-bound form. Despite the changes in the ATPase center, both forms harbor largely ordered L2 regions in essentially identical conformations. These data suggest a unified stimulation mechanism by potassium and calcium because of the existence of a conserved ATPase center promiscuous in binding cations. |
| Starting Page | 8 |
| Ending Page | 14 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.jbc.org/content/281/51/39380.full.pdf |
| PubMed reference number | 17050545v1 |
| Volume Number | 281 |
| Issue Number | 51 |
| Journal | The Journal of biological chemistry |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Adenosine Triphosphate Analog Calcium Chloride Carbamoyl-Phosphate Synthase I Deficiency Disease Cations Crystal Structure DMC1 gene DNA Repair Protein RAD51 Homolog 1 HSP16.5 protein, Methanococcus jannaschii Homologous Recombination Ions Iontophoresis Nucleoproteins Potassium Chloride RAD51 gene inorganic phosphate recombinase |
| Content Type | Text |
| Resource Type | Article |
